Loss of synaptonemal complex protein-1, a synaptonemal complex protein, contributes to the initiation of follicular assembly in the developing rat ovary

Alfonso Paredes, Cecilia Garcia-Rudaz, Bredford Kerr, Veronica Tapia, Gregory Dissen, Maria E. Costa, Anda Cornea, Sergio Ojeda

    Research output: Contribution to journalArticle

    43 Citations (Scopus)

    Abstract

    In the rat ovary, germ and somatic cells become organized into primordial follicles 48-72 h after birth. Although several genes have been implicated in the control of early follicular growth, less is known about the factors involved in the formation of primordial follicles. Using the method of differential display of mRNAs, we found several genes differentially expressed at the time of follicular assembly. One of them encodes synaptonemal complex protein-1 (SCP1), a core component of the protein complex that maintains recombining chromosomes together during prophase I of the first meiotic division in germ cells. This association, evident during the pachytene stage, ends when chromosomal desynapsis begins in the diplotene stage at the end of prophase I. Oocytes become arrested in the diplotene/dictate stage before becoming enclosed into primordial follicles, suggesting that oocytes must complete meiotic prophase I before becoming competent to direct follicle assembly. We now show that attainment of the diplotene stage results in follicular formation. In developing rat ovaries, SCP1mRNAexpression is confined to oocytes and decreases precipitously within 24 h after birth, preceding the organization of primordial follicles. The premature loss of SCP1, achieved via treatment with an antisense oligodeoxynucleotide targeting SCP1 mRNA, resulted in more oocytes reaching the diplotene stage, as evidenced by a decrease in the number of oocytes containing germ cell nuclear antigen-1 (a nuclear protein whose expression ceases in diplotene) and an increase in the number of oocytes expressing MSY2 (a cytoplasmic Y box protein expressed in oocytes that have become arrested in diplotene). SCP1-deficient ovaries exhibited an increased number of newly formed follicles, suggesting that completion of meiotic prophase I endows oocytes with the ability to orchestrate follicular assembly.

    Original languageEnglish (US)
    Pages (from-to)5267-5277
    Number of pages11
    JournalEndocrinology
    Volume146
    Issue number12
    DOIs
    StatePublished - Dec 2005

    Fingerprint

    Synaptonemal Complex
    Meiotic Prophase I
    Oocytes
    Ovary
    Proteins
    Germ Cells
    Pachytene Stage
    Parturition
    Nuclear Antigens
    Oligodeoxyribonucleotides
    Gene Expression Profiling
    Nuclear Proteins
    Genes
    Chromosomes
    Messenger RNA

    ASJC Scopus subject areas

    • Endocrinology
    • Endocrinology, Diabetes and Metabolism

    Cite this

    Loss of synaptonemal complex protein-1, a synaptonemal complex protein, contributes to the initiation of follicular assembly in the developing rat ovary. / Paredes, Alfonso; Garcia-Rudaz, Cecilia; Kerr, Bredford; Tapia, Veronica; Dissen, Gregory; Costa, Maria E.; Cornea, Anda; Ojeda, Sergio.

    In: Endocrinology, Vol. 146, No. 12, 12.2005, p. 5267-5277.

    Research output: Contribution to journalArticle

    Paredes, Alfonso ; Garcia-Rudaz, Cecilia ; Kerr, Bredford ; Tapia, Veronica ; Dissen, Gregory ; Costa, Maria E. ; Cornea, Anda ; Ojeda, Sergio. / Loss of synaptonemal complex protein-1, a synaptonemal complex protein, contributes to the initiation of follicular assembly in the developing rat ovary. In: Endocrinology. 2005 ; Vol. 146, No. 12. pp. 5267-5277.
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