Loss of matrix metalloproteinase 9 activity in Theileria annulata- attenuated cells is at the transcriptional level and is associated with differentially expressed AP-1 species

Rachel Adamson, Mary Logan, Jane Kinnaird, Gordon Langsley, Roger Hall

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The schizont stage of the protozoan parasite Theileria annulata reversibly transforms bovine monocytes into an immortalised and metastatic state. We have been studying T. annulata induction of host matrix metalloproteinases (MMP) which are involved in parasite dissemination and pathogenesis. We have observed that prolonged in vitro culture of T. annulata-infected cell lines results in their attenuation and this process is associated with alterations in both host and parasite gene expression. In particular, a loss in bovine MMP expression in later passage cultures suggests that these parasite-induced MMPs are virulence factors. As a means to further our understanding of the attenuation process we examine in detail the parasite-induced differential expression of one particular bovine proteinase, MMP9, in non-attenuated (p58) and attenuated (p158) passage levels of the Ode vaccine line. We show here that MMP9 expression is regulated at the transcriptional level and we suggest that a particular parasite-induced AP-1 recognition transcription factor present in the Ode non-attenuated line may have a role to play in the expression of this host gene. (C) 2000 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)51-61
Number of pages11
JournalMolecular and Biochemical Parasitology
Volume106
Issue number1
DOIs
StatePublished - Feb 25 2000
Externally publishedYes

Fingerprint

Theileria annulata
Matrix Metalloproteinase 9
Transcription Factor AP-1
Parasites
Matrix Metalloproteinases
Schizonts
Gene Expression
Virulence Factors
Monocytes
Peptide Hydrolases
Vaccines
Cell Line

Keywords

  • Attenuation
  • Matrix metalloproteinase 9
  • Theileria annulata
  • Transcription factor AP-1
  • Transient transfection

ASJC Scopus subject areas

  • Molecular Biology
  • Parasitology

Cite this

Loss of matrix metalloproteinase 9 activity in Theileria annulata- attenuated cells is at the transcriptional level and is associated with differentially expressed AP-1 species. / Adamson, Rachel; Logan, Mary; Kinnaird, Jane; Langsley, Gordon; Hall, Roger.

In: Molecular and Biochemical Parasitology, Vol. 106, No. 1, 25.02.2000, p. 51-61.

Research output: Contribution to journalArticle

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