Loss of human leukocyte antigen expression on colorectal tumor cell lines: Implications for anti-tumor immunity and immunotherapy

M. J. Browning, P. Krausa, A. Rowan, A. B. Hill, D. C. Bicknell, J. G. Bodmer, W. F. Bodmer

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

A system devised for tissue typing the human leukocyte antigen-A (HLA-A) locus from genomic DNA by the polymerase chain reaction (PCR) has been used to investigate abnormalities of HLA expression in a panel of 30 cell lines derived from colorectal adenocarcinomas, by comparison of the HLA-A locus genotype with surface expression of HLA. Eleven cell lines gave single HLA-A locus specificity on PCR typing, suggesting that loss of HLA alleles is a common abnormality. In one of these cell lines the loss of an HLA-A locus allele was confirmed by comparison with DNA from a lymphoblastoid B cell line derived from the same patient. In three cell lines, loss of expression of an HLA-A locus determinant was observed in spite of the presence of the relevant allele in genomic DNA. Three cell lines showed absent HLA expression associated with failure to express β2-microglobulin. These data indicate that at least three independent mechanisms were involved in the loss of HLA expression on the colorectal tumor cell lines.

Original languageEnglish (US)
Pages (from-to)163-168
Number of pages6
JournalJournal of Immunotherapy
Volume14
Issue number3
DOIs
StatePublished - Oct 1993

Keywords

  • Colorectal tumor cell lines
  • Human leukocyte antigen
  • Loss of HLA alleles
  • Polymerase chain reaction

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Pharmacology
  • Cancer Research

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