Long term culture of human tumor cells from malignant effusions as a tool for individualizing chemotherapy

M. M. Lippman, G. L. Gold, Donald (Lynn) Loriaux

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

In order to determine whether long term culture of cells obtained from effusions affords a practical tool for individualizing drug therapy tumor cells were obtained by paracentesis from patient PD with advanced ovarian carcinoma and cultured in medium 199 containing 20% FCS. Nonmalignant cells did not replicate in this system. In primary, and all succeeding generations (> 6 mos.) differentiated organization and function obtain. Two cell types were found; spindle shaped cells devoid of contact inhibition which orient themselves circumferentially around small nests of flat polygonal cells. The latter are characterized by secretion of lipid. In the presence of both cell types 148 pg/ml of β 17 estradiol was found in the medium as compared to 60 pg/ml for equivalent cultures of mouse mammary tumor cells and 30 pg/ml for cell free medium. Assay for other steroids and HCG was negative. Since growth measurement is precise, quantitative drug effects are readily determined. Cell division was blocked by 1 μg/ml adriamycin, but not with hexamethylmelamine, 5 FU, or macromomycin. The patient had received previous courses of HMM and 5 FU so that resistance to these agents may be intrinsic or drug induced. The report demonstrates the practicality of using cell culture from effusions. Recent evidence (Holmes, H.L. and Little, J.M., Lancet, Oct. 26, '74, 985) indicates good correlation between in vitro and in vivo response.

Original languageEnglish (US)
Title of host publicationProceedings of the American Association for Cancer Research
Volume16
Edition66
StatePublished - 1975
Externally publishedYes

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Drug Therapy
Neoplasms
Fluorouracil
Altretamine
Cell Culture Techniques
Contact Inhibition
Paracentesis
Pharmaceutical Preparations
Cell Division
Doxorubicin
Estradiol
Steroids
Breast Neoplasms
Carcinoma
Lipids
Growth

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Lippman, M. M., Gold, G. L., & Loriaux, D. L. (1975). Long term culture of human tumor cells from malignant effusions as a tool for individualizing chemotherapy. In Proceedings of the American Association for Cancer Research (66 ed., Vol. 16)

Long term culture of human tumor cells from malignant effusions as a tool for individualizing chemotherapy. / Lippman, M. M.; Gold, G. L.; Loriaux, Donald (Lynn).

Proceedings of the American Association for Cancer Research. Vol. 16 66. ed. 1975.

Research output: Chapter in Book/Report/Conference proceedingChapter

Lippman, MM, Gold, GL & Loriaux, DL 1975, Long term culture of human tumor cells from malignant effusions as a tool for individualizing chemotherapy. in Proceedings of the American Association for Cancer Research. 66 edn, vol. 16.
Lippman MM, Gold GL, Loriaux DL. Long term culture of human tumor cells from malignant effusions as a tool for individualizing chemotherapy. In Proceedings of the American Association for Cancer Research. 66 ed. Vol. 16. 1975
Lippman, M. M. ; Gold, G. L. ; Loriaux, Donald (Lynn). / Long term culture of human tumor cells from malignant effusions as a tool for individualizing chemotherapy. Proceedings of the American Association for Cancer Research. Vol. 16 66. ed. 1975.
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