Long-term characterization of retinal degeneration in rd1 and rd10 mice using spectral domain optical coherence tomography

Mark Pennesi, Keith V. Michaels, Sienna S. Magee, Anastasiya Maricle, Sean P. Davin, Anupam K. Garg, Michael J. Gale, Daniel Tu, Yuquan Wen, Laura Erker, Peter J. Francis

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Abstract

PURPOSE. We characterize the in vivo changes over time in the retinal structure of wild-type mice alongside two lines of mice deficient in the β-subunit of phosphodiesterase (rd1 and rd10 mice) using spectral domain optical coherence tomography (SD-OCT). METHODS. SD-OCT images were obtained using the Bioptigen spectral domain ophthalmic imaging system (SDOIS). Wild-type C57BL/6J, rd1 and rd10 mice ranging in age from P14 to P206 were sedated with 1% isoflurane. Horizontal and vertical linear scans through the optic nerve, and annular scans around the optic nerve were obtained. RESULTS. SD-OCT imaging of wild-type mice demonstrated visibility of the inner segment/outer segment (IS/OS) junction, external limiting membrane (ELM), outer nuclear layer (ONL), and outer plexiform layer (OPL). At P14, most rd10 mice exhibited normal SD-OCT profiles, but some displayed changes in the IS/OS junction. At the same time point, rd1 mice had severe outer retinal degeneration. In rd10 mice, imaging revealed loss of the IS/OS junction by P18, hyperreflective changes in the ONL at P20, hyperreflective vitreous opacities, and shallow separation of the neural retina from the RPE. Retinal separations were not observed in rd1 mice. Segmentation analysis in wild-type mice demonstrated relatively little variability between animals, while in rd10 and rd1 mice there was a steady decline in outer retinal thickness. Histologic studies demonstrated correlation of retinal features with those seen on SD-OCT scans. Segmentation analysis provides a quantitative and reproducible method for measuring in vivo retinal changes in mice. C demonstrate significantly different features on SD-OCT.

Original languageEnglish (US)
Pages (from-to)4644-4656
Number of pages13
JournalInvestigative Ophthalmology and Visual Science
Volume53
Issue number8
DOIs
StatePublished - Jul 2012

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Retinal Degeneration
Optical Coherence Tomography
Optic Nerve
Isoflurane
Nuclear Envelope
Phosphoric Diester Hydrolases
Retina

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience
  • Medicine(all)

Cite this

Long-term characterization of retinal degeneration in rd1 and rd10 mice using spectral domain optical coherence tomography. / Pennesi, Mark; Michaels, Keith V.; Magee, Sienna S.; Maricle, Anastasiya; Davin, Sean P.; Garg, Anupam K.; Gale, Michael J.; Tu, Daniel; Wen, Yuquan; Erker, Laura; Francis, Peter J.

In: Investigative Ophthalmology and Visual Science, Vol. 53, No. 8, 07.2012, p. 4644-4656.

Research output: Contribution to journalArticle

Pennesi, Mark ; Michaels, Keith V. ; Magee, Sienna S. ; Maricle, Anastasiya ; Davin, Sean P. ; Garg, Anupam K. ; Gale, Michael J. ; Tu, Daniel ; Wen, Yuquan ; Erker, Laura ; Francis, Peter J. / Long-term characterization of retinal degeneration in rd1 and rd10 mice using spectral domain optical coherence tomography. In: Investigative Ophthalmology and Visual Science. 2012 ; Vol. 53, No. 8. pp. 4644-4656.
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T1 - Long-term characterization of retinal degeneration in rd1 and rd10 mice using spectral domain optical coherence tomography

AU - Pennesi, Mark

AU - Michaels, Keith V.

AU - Magee, Sienna S.

AU - Maricle, Anastasiya

AU - Davin, Sean P.

AU - Garg, Anupam K.

AU - Gale, Michael J.

AU - Tu, Daniel

AU - Wen, Yuquan

AU - Erker, Laura

AU - Francis, Peter J.

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N2 - PURPOSE. We characterize the in vivo changes over time in the retinal structure of wild-type mice alongside two lines of mice deficient in the β-subunit of phosphodiesterase (rd1 and rd10 mice) using spectral domain optical coherence tomography (SD-OCT). METHODS. SD-OCT images were obtained using the Bioptigen spectral domain ophthalmic imaging system (SDOIS). Wild-type C57BL/6J, rd1 and rd10 mice ranging in age from P14 to P206 were sedated with 1% isoflurane. Horizontal and vertical linear scans through the optic nerve, and annular scans around the optic nerve were obtained. RESULTS. SD-OCT imaging of wild-type mice demonstrated visibility of the inner segment/outer segment (IS/OS) junction, external limiting membrane (ELM), outer nuclear layer (ONL), and outer plexiform layer (OPL). At P14, most rd10 mice exhibited normal SD-OCT profiles, but some displayed changes in the IS/OS junction. At the same time point, rd1 mice had severe outer retinal degeneration. In rd10 mice, imaging revealed loss of the IS/OS junction by P18, hyperreflective changes in the ONL at P20, hyperreflective vitreous opacities, and shallow separation of the neural retina from the RPE. Retinal separations were not observed in rd1 mice. Segmentation analysis in wild-type mice demonstrated relatively little variability between animals, while in rd10 and rd1 mice there was a steady decline in outer retinal thickness. Histologic studies demonstrated correlation of retinal features with those seen on SD-OCT scans. Segmentation analysis provides a quantitative and reproducible method for measuring in vivo retinal changes in mice. C demonstrate significantly different features on SD-OCT.

AB - PURPOSE. We characterize the in vivo changes over time in the retinal structure of wild-type mice alongside two lines of mice deficient in the β-subunit of phosphodiesterase (rd1 and rd10 mice) using spectral domain optical coherence tomography (SD-OCT). METHODS. SD-OCT images were obtained using the Bioptigen spectral domain ophthalmic imaging system (SDOIS). Wild-type C57BL/6J, rd1 and rd10 mice ranging in age from P14 to P206 were sedated with 1% isoflurane. Horizontal and vertical linear scans through the optic nerve, and annular scans around the optic nerve were obtained. RESULTS. SD-OCT imaging of wild-type mice demonstrated visibility of the inner segment/outer segment (IS/OS) junction, external limiting membrane (ELM), outer nuclear layer (ONL), and outer plexiform layer (OPL). At P14, most rd10 mice exhibited normal SD-OCT profiles, but some displayed changes in the IS/OS junction. At the same time point, rd1 mice had severe outer retinal degeneration. In rd10 mice, imaging revealed loss of the IS/OS junction by P18, hyperreflective changes in the ONL at P20, hyperreflective vitreous opacities, and shallow separation of the neural retina from the RPE. Retinal separations were not observed in rd1 mice. Segmentation analysis in wild-type mice demonstrated relatively little variability between animals, while in rd10 and rd1 mice there was a steady decline in outer retinal thickness. Histologic studies demonstrated correlation of retinal features with those seen on SD-OCT scans. Segmentation analysis provides a quantitative and reproducible method for measuring in vivo retinal changes in mice. C demonstrate significantly different features on SD-OCT.

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