Long-range energy transfer on a DNA substrate

C. R. Guest, S. M. Chen, Fred Heffron, D. P. Millar

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The technique of long-range electronic energy transfer has been used to obtain structural data on nucleic acids in solution. Fluorescent chromophores have been covalently attached via an aminoethyl linker to the 5'-terminus of a synthetic oligonucleotide. Duplexes were formed with an energy donor, fluorescein, at one end and an acceptor, tetramethyl rhodamine, at the other. The rate of energy transfer has been determined from the fluorescence decay of the donor which was measured with a picosecond photon counting system. The effects of dye: DNA interactions cause additional quenching of the donor fluorescence and must be considered in the interpretation of the energy transfer experiments. An apparent donor:acceptor distance of 40.9 angstrom was calculated for a 12 b.p. labelled duplex.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsJoseph F. Lakowicz
PublisherPubl by Int Soc for Optical Engineering
Pages663-668
Number of pages6
Volume1204 pt 2
ISBN (Print)0819402451
Publication statusPublished - 1990
Externally publishedYes
EventTime-Resolved Laser Spectroscopy in Biochemistry II - Los Angeles, CA, USA
Duration: Jan 15 1990Jan 17 1990

Other

OtherTime-Resolved Laser Spectroscopy in Biochemistry II
CityLos Angeles, CA, USA
Period1/15/901/17/90

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ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

Guest, C. R., Chen, S. M., Heffron, F., & Millar, D. P. (1990). Long-range energy transfer on a DNA substrate. In J. F. Lakowicz (Ed.), Proceedings of SPIE - The International Society for Optical Engineering (Vol. 1204 pt 2, pp. 663-668). Publ by Int Soc for Optical Engineering.