Long-range energy transfer on a DNA substrate

C. R. Guest, S. M. Chen, F. E. Heffron, D. P. Millar

    Research output: Chapter in Book/Report/Conference proceedingConference contribution


    The technique of long-range electronic energy transfer has been used to obtain structural data on nucleic acids in solution. Fluorescent chromophores have been covalently attached via an aminoethyl linker to the 5'-terminus of a synthetic oligonucleotide. Duplexes were formed with an energy donor, fluorescein, at one end and an acceptor, tetramethyl rhodamine, at the other. The rate of energy transfer has been determined from the fluorescence decay of the donor which was measured with a picosecond photon counting system. The effects of dye: DNA interactions cause additional quenching of the donor fluorescence and must be considered in the interpretation of the energy transfer experiments. An apparent donor:acceptor distance of 40.9 angstrom was calculated for a 12 b.p. labelled duplex.

    Original languageEnglish (US)
    Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
    EditorsJoseph F. Lakowicz
    PublisherPubl by Int Soc for Optical Engineering
    Number of pages6
    ISBN (Print)0819402451
    StatePublished - Dec 1 1990
    EventTime-Resolved Laser Spectroscopy in Biochemistry II - Los Angeles, CA, USA
    Duration: Jan 15 1990Jan 17 1990

    Publication series

    NameProceedings of SPIE - The International Society for Optical Engineering
    Volume1204 pt 2
    ISSN (Print)0277-786X


    OtherTime-Resolved Laser Spectroscopy in Biochemistry II
    CityLos Angeles, CA, USA

    ASJC Scopus subject areas

    • Electronic, Optical and Magnetic Materials
    • Condensed Matter Physics
    • Computer Science Applications
    • Applied Mathematics
    • Electrical and Electronic Engineering


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