Locations and molecular forms of gastrin-releasing peptide-like immunoreactive entities in ovine pregnancy

Q. Xiao, J. R G Challis, M. Fraser, M. E. Wlodek, G. D. Thorburn, F. Cuttita, D. J. Hill, S. St.-Pierre, Eliot Spindel, T. J. McDonald

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    8 Scopus citations

    Abstract

    Large quantities of gastrin-releasing peptide-like immunoreactivity (GRP- LI) are present in ovine pregnancy fluids (allantoic fluid > fetal plasma > esophageal fluid = amniotic fluid = urine > maternal plasma) and in term endometrium (60 ± 29 pmol · g-1) and myometrium (4.5 ± 1.2 pmol · g- 1). The larger molecular size [greater than GRP(1-27)] of this GRP-LI entity is not due to a GRP binding protein nor to a C terminal extension of GRP. In contrast, ovine fetal colon extracts appear to contain the usual GRP(1-27) and GRP(18-27) forms. Hence, the uterus, not the fetus, is the probable source of this novel GRP-like peptide. It apparently acts as a hormone in ovine pregnancy and may play an important role in fetal-placental development.

    Original languageEnglish (US)
    Pages (from-to)489-495
    Number of pages7
    JournalPeptides
    Volume17
    Issue number3
    DOIs
    Publication statusPublished - 1996

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    Keywords

    • Bombesin
    • Gastrin-releasing peptide
    • Gel filtration
    • Ovine pregnancy
    • Reverse-phase HPLC
    • Uterus

    ASJC Scopus subject areas

    • Biochemistry
    • Endocrinology
    • Physiology
    • Cellular and Molecular Neuroscience

    Cite this

    Xiao, Q., Challis, J. R. G., Fraser, M., Wlodek, M. E., Thorburn, G. D., Cuttita, F., ... McDonald, T. J. (1996). Locations and molecular forms of gastrin-releasing peptide-like immunoreactive entities in ovine pregnancy. Peptides, 17(3), 489-495. https://doi.org/10.1016/0196-9781(96)00003-4