Locations and molecular forms of gastrin-releasing peptide-like immunoreactive entities in ovine pregnancy

Q. Xiao, J. R.G. Challis, M. Fraser, M. E. Wlodek, G. D. Thorburn, F. Cuttita, D. J. Hill, S. St.-Pierre, E. R. Spindel, T. J. McDonald

    Research output: Contribution to journalArticle

    8 Scopus citations

    Abstract

    Large quantities of gastrin-releasing peptide-like immunoreactivity (GRP- LI) are present in ovine pregnancy fluids (allantoic fluid > fetal plasma > esophageal fluid = amniotic fluid = urine > maternal plasma) and in term endometrium (60 ± 29 pmol · g-1) and myometrium (4.5 ± 1.2 pmol · g- 1). The larger molecular size [greater than GRP(1-27)] of this GRP-LI entity is not due to a GRP binding protein nor to a C terminal extension of GRP. In contrast, ovine fetal colon extracts appear to contain the usual GRP(1-27) and GRP(18-27) forms. Hence, the uterus, not the fetus, is the probable source of this novel GRP-like peptide. It apparently acts as a hormone in ovine pregnancy and may play an important role in fetal-placental development.

    Original languageEnglish (US)
    Pages (from-to)489-495
    Number of pages7
    JournalPeptides
    Volume17
    Issue number3
    DOIs
    StatePublished - Jan 1 1996

    Keywords

    • Bombesin
    • Gastrin-releasing peptide
    • Gel filtration
    • Ovine pregnancy
    • Reverse-phase HPLC
    • Uterus

    ASJC Scopus subject areas

    • Biochemistry
    • Physiology
    • Endocrinology
    • Cellular and Molecular Neuroscience

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  • Cite this

    Xiao, Q., Challis, J. R. G., Fraser, M., Wlodek, M. E., Thorburn, G. D., Cuttita, F., Hill, D. J., St.-Pierre, S., Spindel, E. R., & McDonald, T. J. (1996). Locations and molecular forms of gastrin-releasing peptide-like immunoreactive entities in ovine pregnancy. Peptides, 17(3), 489-495. https://doi.org/10.1016/0196-9781(96)00003-4