Localization to chromosome 10 of a locus influencing morphine analgesia in crosses derived from C57BL/ and DBA/2 strains

J. K. Belknap; J. S. Mogil; M. L. Helms; S. P. Richards; L. A. O'Toole; S. E. Bergeson; K. J. Buck

doi:10.1016/0024-3205(95)02040-P

Localization to chromosome 10 of a locus influencing morphine analgesia in crosses derived from C57BL/ and DBA/2 strains

J. K. Belknap, J. S. Mogil, M. L. Helms, S. P. Richards, L. A. O'Toole, S. E. Bergeson, K. J. Buck

Behavioral Neuroscience

Research output: Contribution to journal › Article › peer-review

97 Scopus citations

Abstract

A quantitative trait locus (QTL) was detected and mapped to proximal chromosome 10 near the markers Mpmv5 and D10Mit51 with a strong influence on morphine-induced analgesia in the BXD recombinant inbred (Rl) strains and in an F₂ cross (B6D2F2) between the BXD progenitor strains, C57BL/6 and DBA/2. A LOD score of 3.9 (p <. 00002) was seen for analgesia using the hot plate assay. Naloxone Bmax was also associated with this chromosome region in BXD RI mice. The mu opioid receptor gene (Oprm) has recently been mapped to this same chromosome region. The observation that several morphine-related traits and naloxone Bmax appear to be partly determined by this presumed single locus is consistent with the hypothesis that the mu opioid receptor gene, or one of its modulators, is the basis for the QTL.

Original language	English (US)
Pages (from-to)	PL117-PL124
Journal	Life Sciences
Volume	57
Issue number	10
DOIs	https://doi.org/10.1016/0024-3205(95)02040-P
State	Published - Jul 28 1995

Keywords

BXD
C57BL/6
DBA/2
QTL mapping
analgesia
opioid receptors

ASJC Scopus subject areas

General Pharmacology, Toxicology and Pharmaceutics
General Biochemistry, Genetics and Molecular Biology

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10.1016/0024-3205(95)02040-P

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@article{30ce46b911344bdcaf41e5bbd077c77b,

title = "Localization to chromosome 10 of a locus influencing morphine analgesia in crosses derived from C57BL/ and DBA/2 strains",

abstract = "A quantitative trait locus (QTL) was detected and mapped to proximal chromosome 10 near the markers Mpmv5 and D10Mit51 with a strong influence on morphine-induced analgesia in the BXD recombinant inbred (Rl) strains and in an F2 cross (B6D2F2) between the BXD progenitor strains, C57BL/6 and DBA/2. A LOD score of 3.9 (p <. 00002) was seen for analgesia using the hot plate assay. Naloxone Bmax was also associated with this chromosome region in BXD RI mice. The mu opioid receptor gene (Oprm) has recently been mapped to this same chromosome region. The observation that several morphine-related traits and naloxone Bmax appear to be partly determined by this presumed single locus is consistent with the hypothesis that the mu opioid receptor gene, or one of its modulators, is the basis for the QTL.",

keywords = "BXD, C57BL/6, DBA/2, QTL mapping, analgesia, opioid receptors",

author = "Belknap, {J. K.} and Mogil, {J. S.} and Helms, {M. L.} and Richards, {S. P.} and O'Toole, {L. A.} and Bergeson, {S. E.} and Buck, {K. J.}",

note = "Funding Information: This work was supported by a Merit Review program from the Department of Veterans Affairs, NIH Grant DA05228 and NIDA Contracts 271-87-8020, 271-90-7405. Appreciation is extended to Dr. Steve R. Mitchell for expert assistance with the BXD QTL analyses.",

year = "1995",

month = jul,

day = "28",

doi = "10.1016/0024-3205(95)02040-P",

language = "English (US)",

volume = "57",

pages = "PL117--PL124",

journal = "Life Sciences",

issn = "0024-3205",

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T1 - Localization to chromosome 10 of a locus influencing morphine analgesia in crosses derived from C57BL/ and DBA/2 strains

AU - Belknap, J. K.

AU - Mogil, J. S.

AU - Helms, M. L.

AU - Richards, S. P.

AU - O'Toole, L. A.

AU - Bergeson, S. E.

AU - Buck, K. J.

N1 - Funding Information: This work was supported by a Merit Review program from the Department of Veterans Affairs, NIH Grant DA05228 and NIDA Contracts 271-87-8020, 271-90-7405. Appreciation is extended to Dr. Steve R. Mitchell for expert assistance with the BXD QTL analyses.

PY - 1995/7/28

Y1 - 1995/7/28

N2 - A quantitative trait locus (QTL) was detected and mapped to proximal chromosome 10 near the markers Mpmv5 and D10Mit51 with a strong influence on morphine-induced analgesia in the BXD recombinant inbred (Rl) strains and in an F2 cross (B6D2F2) between the BXD progenitor strains, C57BL/6 and DBA/2. A LOD score of 3.9 (p <. 00002) was seen for analgesia using the hot plate assay. Naloxone Bmax was also associated with this chromosome region in BXD RI mice. The mu opioid receptor gene (Oprm) has recently been mapped to this same chromosome region. The observation that several morphine-related traits and naloxone Bmax appear to be partly determined by this presumed single locus is consistent with the hypothesis that the mu opioid receptor gene, or one of its modulators, is the basis for the QTL.

AB - A quantitative trait locus (QTL) was detected and mapped to proximal chromosome 10 near the markers Mpmv5 and D10Mit51 with a strong influence on morphine-induced analgesia in the BXD recombinant inbred (Rl) strains and in an F2 cross (B6D2F2) between the BXD progenitor strains, C57BL/6 and DBA/2. A LOD score of 3.9 (p <. 00002) was seen for analgesia using the hot plate assay. Naloxone Bmax was also associated with this chromosome region in BXD RI mice. The mu opioid receptor gene (Oprm) has recently been mapped to this same chromosome region. The observation that several morphine-related traits and naloxone Bmax appear to be partly determined by this presumed single locus is consistent with the hypothesis that the mu opioid receptor gene, or one of its modulators, is the basis for the QTL.

KW - BXD

KW - C57BL/6

KW - DBA/2

KW - QTL mapping

KW - analgesia

KW - opioid receptors

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Localization to chromosome 10 of a locus influencing morphine analgesia in crosses derived from C57BL/ and DBA/2 strains

Abstract

Keywords

ASJC Scopus subject areas

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