Abstract
The distribution of TNF-α, p55 TNF receptor (TNF-R) and p75 TNF-R in normal skin and uninvolved and lesional skin from psoriasis patients has been investigated, using specific mono- and polyclonal antibodies. In normal skin, and uninvolved and lesional skin from psoriasis patients, p55 TNF-R is associated with epidermal keratinocytes and a network of upper dermal dendritic cells. This suggests that the actions of TNF-α on epidermal cells in vivo are mediated by binding to the p55 TNF-R. In lesional psoriasis skin, there was staining of the parakeratotic stratum corneum and increased expression of p55 TNF-R in association with upper dermal blood vessels. Staining for p75 TNF-R in normal skin was restricted to eccrine sweat ducts and dermal dendritic cells, and was absent from the epidermis. In lesional psoriasis skin, there was staining for p75 TNF-R in association with upper dermal blood vessels and perivascular infiltrating cells. TNF-α in normal skin was predominantly localized to the basal cell layers of the epidermis, and was seen in association with eccrine ducts and sebaceous glands. In lesional psoriasis skin, and to a lesser extent in uninvolved psoriasis skin, TNF-α was distributed throughout the epidermis, and was also specifically localized to upper dermal blood vessels. Up-regulation of TNF-α, p55 TNF-R and p75 TNF-R on dermal blood vessels in psoriasis may play an important role in the pathogenesis of this condition by promoting cutaneous recruitment of inflammatory cells.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 354-362 |
| Number of pages | 9 |
| Journal | Clinical and Experimental Immunology |
| Volume | 94 |
| Issue number | 2 |
| State | Published - 1993 |
| Externally published | Yes |
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Keywords
- psoriasis
- skin
- TNF receptors
- TNF-α
ASJC Scopus subject areas
- Immunology
Cite this
Localization of tumour necrosis factor-alpha (TNF-α) and its receptors in normal and psoriatic skin : Epidermal cells express the 55-kD but not the 75-kD TNF receptor. / Kristensen, M.; Chu, Cong-Qiu; Eedy, D. J.; Feldmann, M.; Brennan, F. M.; Breathnach, S. M.
In: Clinical and Experimental Immunology, Vol. 94, No. 2, 1993, p. 354-362.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Localization of tumour necrosis factor-alpha (TNF-α) and its receptors in normal and psoriatic skin
T2 - Epidermal cells express the 55-kD but not the 75-kD TNF receptor
AU - Kristensen, M.
AU - Chu, Cong-Qiu
AU - Eedy, D. J.
AU - Feldmann, M.
AU - Brennan, F. M.
AU - Breathnach, S. M.
PY - 1993
Y1 - 1993
N2 - The distribution of TNF-α, p55 TNF receptor (TNF-R) and p75 TNF-R in normal skin and uninvolved and lesional skin from psoriasis patients has been investigated, using specific mono- and polyclonal antibodies. In normal skin, and uninvolved and lesional skin from psoriasis patients, p55 TNF-R is associated with epidermal keratinocytes and a network of upper dermal dendritic cells. This suggests that the actions of TNF-α on epidermal cells in vivo are mediated by binding to the p55 TNF-R. In lesional psoriasis skin, there was staining of the parakeratotic stratum corneum and increased expression of p55 TNF-R in association with upper dermal blood vessels. Staining for p75 TNF-R in normal skin was restricted to eccrine sweat ducts and dermal dendritic cells, and was absent from the epidermis. In lesional psoriasis skin, there was staining for p75 TNF-R in association with upper dermal blood vessels and perivascular infiltrating cells. TNF-α in normal skin was predominantly localized to the basal cell layers of the epidermis, and was seen in association with eccrine ducts and sebaceous glands. In lesional psoriasis skin, and to a lesser extent in uninvolved psoriasis skin, TNF-α was distributed throughout the epidermis, and was also specifically localized to upper dermal blood vessels. Up-regulation of TNF-α, p55 TNF-R and p75 TNF-R on dermal blood vessels in psoriasis may play an important role in the pathogenesis of this condition by promoting cutaneous recruitment of inflammatory cells.
AB - The distribution of TNF-α, p55 TNF receptor (TNF-R) and p75 TNF-R in normal skin and uninvolved and lesional skin from psoriasis patients has been investigated, using specific mono- and polyclonal antibodies. In normal skin, and uninvolved and lesional skin from psoriasis patients, p55 TNF-R is associated with epidermal keratinocytes and a network of upper dermal dendritic cells. This suggests that the actions of TNF-α on epidermal cells in vivo are mediated by binding to the p55 TNF-R. In lesional psoriasis skin, there was staining of the parakeratotic stratum corneum and increased expression of p55 TNF-R in association with upper dermal blood vessels. Staining for p75 TNF-R in normal skin was restricted to eccrine sweat ducts and dermal dendritic cells, and was absent from the epidermis. In lesional psoriasis skin, there was staining for p75 TNF-R in association with upper dermal blood vessels and perivascular infiltrating cells. TNF-α in normal skin was predominantly localized to the basal cell layers of the epidermis, and was seen in association with eccrine ducts and sebaceous glands. In lesional psoriasis skin, and to a lesser extent in uninvolved psoriasis skin, TNF-α was distributed throughout the epidermis, and was also specifically localized to upper dermal blood vessels. Up-regulation of TNF-α, p55 TNF-R and p75 TNF-R on dermal blood vessels in psoriasis may play an important role in the pathogenesis of this condition by promoting cutaneous recruitment of inflammatory cells.
KW - psoriasis
KW - skin
KW - TNF receptors
KW - TNF-α
UR - http://www.scopus.com/inward/record.url?scp=0027490541&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027490541&partnerID=8YFLogxK
M3 - Article
VL - 94
SP - 354
EP - 362
JO - Clinical and Experimental Immunology
JF - Clinical and Experimental Immunology
SN - 0009-9104
IS - 2
ER -