Localization of tumour necrosis factor-alpha (TNF-α) and its receptors in normal and psoriatic skin

Epidermal cells express the 55-kD but not the 75-kD TNF receptor

M. Kristensen, Cong-Qiu Chu, D. J. Eedy, M. Feldmann, F. M. Brennan, S. M. Breathnach

Research output: Contribution to journalArticle

144 Citations (Scopus)

Abstract

The distribution of TNF-α, p55 TNF receptor (TNF-R) and p75 TNF-R in normal skin and uninvolved and lesional skin from psoriasis patients has been investigated, using specific mono- and polyclonal antibodies. In normal skin, and uninvolved and lesional skin from psoriasis patients, p55 TNF-R is associated with epidermal keratinocytes and a network of upper dermal dendritic cells. This suggests that the actions of TNF-α on epidermal cells in vivo are mediated by binding to the p55 TNF-R. In lesional psoriasis skin, there was staining of the parakeratotic stratum corneum and increased expression of p55 TNF-R in association with upper dermal blood vessels. Staining for p75 TNF-R in normal skin was restricted to eccrine sweat ducts and dermal dendritic cells, and was absent from the epidermis. In lesional psoriasis skin, there was staining for p75 TNF-R in association with upper dermal blood vessels and perivascular infiltrating cells. TNF-α in normal skin was predominantly localized to the basal cell layers of the epidermis, and was seen in association with eccrine ducts and sebaceous glands. In lesional psoriasis skin, and to a lesser extent in uninvolved psoriasis skin, TNF-α was distributed throughout the epidermis, and was also specifically localized to upper dermal blood vessels. Up-regulation of TNF-α, p55 TNF-R and p75 TNF-R on dermal blood vessels in psoriasis may play an important role in the pathogenesis of this condition by promoting cutaneous recruitment of inflammatory cells.

Original languageEnglish (US)
Pages (from-to)354-362
Number of pages9
JournalClinical and Experimental Immunology
Volume94
Issue number2
StatePublished - 1993
Externally publishedYes

Fingerprint

Tumor Necrosis Factor Receptors
Tumor Necrosis Factor-alpha
Skin
Psoriasis
Blood Vessels
Epidermis
Langerhans Cells
Staining and Labeling
Eccrine Glands
Sebaceous Glands
Sweat
Keratinocytes
Cornea

Keywords

  • psoriasis
  • skin
  • TNF receptors
  • TNF-α

ASJC Scopus subject areas

  • Immunology

Cite this

Localization of tumour necrosis factor-alpha (TNF-α) and its receptors in normal and psoriatic skin : Epidermal cells express the 55-kD but not the 75-kD TNF receptor. / Kristensen, M.; Chu, Cong-Qiu; Eedy, D. J.; Feldmann, M.; Brennan, F. M.; Breathnach, S. M.

In: Clinical and Experimental Immunology, Vol. 94, No. 2, 1993, p. 354-362.

Research output: Contribution to journalArticle

Kristensen, M. ; Chu, Cong-Qiu ; Eedy, D. J. ; Feldmann, M. ; Brennan, F. M. ; Breathnach, S. M. / Localization of tumour necrosis factor-alpha (TNF-α) and its receptors in normal and psoriatic skin : Epidermal cells express the 55-kD but not the 75-kD TNF receptor. In: Clinical and Experimental Immunology. 1993 ; Vol. 94, No. 2. pp. 354-362.
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abstract = "The distribution of TNF-α, p55 TNF receptor (TNF-R) and p75 TNF-R in normal skin and uninvolved and lesional skin from psoriasis patients has been investigated, using specific mono- and polyclonal antibodies. In normal skin, and uninvolved and lesional skin from psoriasis patients, p55 TNF-R is associated with epidermal keratinocytes and a network of upper dermal dendritic cells. This suggests that the actions of TNF-α on epidermal cells in vivo are mediated by binding to the p55 TNF-R. In lesional psoriasis skin, there was staining of the parakeratotic stratum corneum and increased expression of p55 TNF-R in association with upper dermal blood vessels. Staining for p75 TNF-R in normal skin was restricted to eccrine sweat ducts and dermal dendritic cells, and was absent from the epidermis. In lesional psoriasis skin, there was staining for p75 TNF-R in association with upper dermal blood vessels and perivascular infiltrating cells. TNF-α in normal skin was predominantly localized to the basal cell layers of the epidermis, and was seen in association with eccrine ducts and sebaceous glands. In lesional psoriasis skin, and to a lesser extent in uninvolved psoriasis skin, TNF-α was distributed throughout the epidermis, and was also specifically localized to upper dermal blood vessels. Up-regulation of TNF-α, p55 TNF-R and p75 TNF-R on dermal blood vessels in psoriasis may play an important role in the pathogenesis of this condition by promoting cutaneous recruitment of inflammatory cells.",
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