Localization of thiazide-sensitive Na+-Cl- cotransport and associated gene products in mouse DCT

Valentina Câmpean, Jörn Kricke, David Ellison, Friedrich C. Luft, Sebastian Bachmann

Research output: Contribution to journalArticle

79 Scopus citations

Abstract

The mammalian distal nephron develops a complex assembly of specialized cell types to accomplish the fine adjustment of urinary electrolyte composition. The epithelia of the distal convoluted tubule (DCT), the connecting tubule (CNT), and the cortical collecting duct (CCD) show an axial structural heterogeneity that has been functionally elucidated by the localization of proteins involved in transepithelial ion transport. We compared the distribution of the thiazide-sensitive Na+-Cl- cotransporter (TSC), basolateral Na+/Ca2+ exchanger (Na/Ca), cytosolic calcium-binding proteins calbindin D2SK and parvalbumin, and the key enzyme for selective aldosterone actions, 11βhydroxysteroid-dehydrogenase 2 (11HSD2), in the distal convolutions of the mouse. In the mouse, as opposed to the rat, we found no clear subsegmentation of the DCT into a proximal (DCT1) and a distal (DCT2) portion. The TSC was expressed along the entire DCT. Na/Ca and calbindin D28K were similarly expressed along most of the DCT, with minor exceptions in the initial portion of the DCT. Both were also present in the CNT. Parvalbumin was found in the entire DCT, with an occasional absence from short end portions of the DCT, and was not present in CNT. 11HSD2 was predominantly located in the CNT and CCD. Short end portions of DCT only occasionally showed the 11HSD2 signal. We also observed an overlap of 11HSD2 immunoreactivity and mRNA staining. Our observations will have implications in understanding the physiological effects of gene disruption and targeting experiments in the mouse.

Original languageEnglish (US)
Pages (from-to)F1028-F1035
JournalAmerican Journal of Physiology - Renal Physiology
Volume281
Issue number6 50-6
DOIs
StatePublished - 2001

Keywords

  • 11β-hydroxysteroid-dehydrogenase type 2
  • Calbindin
  • Connecting tubule
  • Distal convoluted tubule
  • Parvalbumin
  • Sodium-calcium exchanger

ASJC Scopus subject areas

  • Physiology
  • Urology

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