Localization of the fibrillin (FBN) gene to chromosome 15, band q21.1

R. Ellen Magenis, Cheryl Maslen, Leslie Smith, Leland Allen, Lynn Sakai

Research output: Contribution to journalArticle

101 Citations (Scopus)

Abstract

Fibrillin (FBN), a large extracellular matrix glycoprotein, is an important component of structures called microfibrils. Because fibrillin microfibrils appear to be abnormal in patients with the Marfan syndrome, fibrillin is a candidate for the gene defect in the Marfan syndrome. Derived clones from fibrillin cDNA were used as probes in isotopic and nonisotopic in situ hybridization studies to map the chromosomal location of the fibrillin gene. Fluorescent signals were found on chromosome 15 band q21.1; an excess of silver grains was noted over a similar region of chromosome 15 following in situ hybridization with a tritium-labeled probe. These results are consistent with linkage studies that localize the Marfan gene to chromosome 15.

Original languageEnglish (US)
Pages (from-to)346-351
Number of pages6
JournalGenomics
Volume11
Issue number2
DOIs
StatePublished - 1991

Fingerprint

Chromosomes, Human, Pair 15
Microfibrils
Marfan Syndrome
Genes
In Situ Hybridization
Tritium
Silver
Extracellular Matrix
Glycoproteins
Complementary DNA
Clone Cells
Fibrillins

ASJC Scopus subject areas

  • Genetics

Cite this

Localization of the fibrillin (FBN) gene to chromosome 15, band q21.1. / Magenis, R. Ellen; Maslen, Cheryl; Smith, Leslie; Allen, Leland; Sakai, Lynn.

In: Genomics, Vol. 11, No. 2, 1991, p. 346-351.

Research output: Contribution to journalArticle

Magenis, R. Ellen ; Maslen, Cheryl ; Smith, Leslie ; Allen, Leland ; Sakai, Lynn. / Localization of the fibrillin (FBN) gene to chromosome 15, band q21.1. In: Genomics. 1991 ; Vol. 11, No. 2. pp. 346-351.
@article{c20dbdfa666445aebb6f6b998e3639ae,
title = "Localization of the fibrillin (FBN) gene to chromosome 15, band q21.1",
abstract = "Fibrillin (FBN), a large extracellular matrix glycoprotein, is an important component of structures called microfibrils. Because fibrillin microfibrils appear to be abnormal in patients with the Marfan syndrome, fibrillin is a candidate for the gene defect in the Marfan syndrome. Derived clones from fibrillin cDNA were used as probes in isotopic and nonisotopic in situ hybridization studies to map the chromosomal location of the fibrillin gene. Fluorescent signals were found on chromosome 15 band q21.1; an excess of silver grains was noted over a similar region of chromosome 15 following in situ hybridization with a tritium-labeled probe. These results are consistent with linkage studies that localize the Marfan gene to chromosome 15.",
author = "Magenis, {R. Ellen} and Cheryl Maslen and Leslie Smith and Leland Allen and Lynn Sakai",
year = "1991",
doi = "10.1016/0888-7543(91)90142-2",
language = "English (US)",
volume = "11",
pages = "346--351",
journal = "Genomics",
issn = "0888-7543",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Localization of the fibrillin (FBN) gene to chromosome 15, band q21.1

AU - Magenis, R. Ellen

AU - Maslen, Cheryl

AU - Smith, Leslie

AU - Allen, Leland

AU - Sakai, Lynn

PY - 1991

Y1 - 1991

N2 - Fibrillin (FBN), a large extracellular matrix glycoprotein, is an important component of structures called microfibrils. Because fibrillin microfibrils appear to be abnormal in patients with the Marfan syndrome, fibrillin is a candidate for the gene defect in the Marfan syndrome. Derived clones from fibrillin cDNA were used as probes in isotopic and nonisotopic in situ hybridization studies to map the chromosomal location of the fibrillin gene. Fluorescent signals were found on chromosome 15 band q21.1; an excess of silver grains was noted over a similar region of chromosome 15 following in situ hybridization with a tritium-labeled probe. These results are consistent with linkage studies that localize the Marfan gene to chromosome 15.

AB - Fibrillin (FBN), a large extracellular matrix glycoprotein, is an important component of structures called microfibrils. Because fibrillin microfibrils appear to be abnormal in patients with the Marfan syndrome, fibrillin is a candidate for the gene defect in the Marfan syndrome. Derived clones from fibrillin cDNA were used as probes in isotopic and nonisotopic in situ hybridization studies to map the chromosomal location of the fibrillin gene. Fluorescent signals were found on chromosome 15 band q21.1; an excess of silver grains was noted over a similar region of chromosome 15 following in situ hybridization with a tritium-labeled probe. These results are consistent with linkage studies that localize the Marfan gene to chromosome 15.

UR - http://www.scopus.com/inward/record.url?scp=0026002215&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026002215&partnerID=8YFLogxK

U2 - 10.1016/0888-7543(91)90142-2

DO - 10.1016/0888-7543(91)90142-2

M3 - Article

C2 - 1769651

AN - SCOPUS:0026002215

VL - 11

SP - 346

EP - 351

JO - Genomics

JF - Genomics

SN - 0888-7543

IS - 2

ER -