Localization of 125I-insulin binding sites in the rat hypothalamus by quantitative autoradiography

Eric S. Corp, Stephen C. Woods, Daniel Porte, Daniel Dorsa, Dianne P. Figlewicz, Denis G. Baskin

Research output: Contribution to journalArticle

130 Citations (Scopus)

Abstract

In vitro autoradiography and computer video densitometry were used to localize and quantify binding of 125I-insulin in the hypothalamus of the rat brain. Highest specific binding was found in the arcuate. dorsomedial, suprachiasmatic, paraventricular and periventricular regions. Significantly lower binding was present in the ventromedial nucleus and median eminence. The results are consistent with the hypothesis that insulin modulates the neural regulation of feeding by acting at sites in the hypothalamus.

Original languageEnglish (US)
Pages (from-to)17-22
Number of pages6
JournalNeuroscience Letters
Volume70
Issue number1
DOIs
StatePublished - Sep 25 1986
Externally publishedYes

Fingerprint

Autoradiography
Hypothalamus
Binding Sites
Insulin
Median Eminence
Densitometry
Brain
In Vitro Techniques

Keywords

  • Autoradiography
  • Brain
  • Feeding
  • Insulin
  • Rat
  • Receptor

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Localization of 125I-insulin binding sites in the rat hypothalamus by quantitative autoradiography. / Corp, Eric S.; Woods, Stephen C.; Porte, Daniel; Dorsa, Daniel; Figlewicz, Dianne P.; Baskin, Denis G.

In: Neuroscience Letters, Vol. 70, No. 1, 25.09.1986, p. 17-22.

Research output: Contribution to journalArticle

Corp, Eric S. ; Woods, Stephen C. ; Porte, Daniel ; Dorsa, Daniel ; Figlewicz, Dianne P. ; Baskin, Denis G. / Localization of 125I-insulin binding sites in the rat hypothalamus by quantitative autoradiography. In: Neuroscience Letters. 1986 ; Vol. 70, No. 1. pp. 17-22.
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