Localization of 125I-insulin binding sites in the rat hypothalamus by quantitative autoradiography

Eric S. Corp, Stephen C. Woods, Daniel Porte, Daniel M. Dorsa, Dianne P. Figlewicz, Denis G. Baskin

Research output: Contribution to journalArticle

132 Scopus citations

Abstract

In vitro autoradiography and computer video densitometry were used to localize and quantify binding of 125I-insulin in the hypothalamus of the rat brain. Highest specific binding was found in the arcuate. dorsomedial, suprachiasmatic, paraventricular and periventricular regions. Significantly lower binding was present in the ventromedial nucleus and median eminence. The results are consistent with the hypothesis that insulin modulates the neural regulation of feeding by acting at sites in the hypothalamus.

Original languageEnglish (US)
Pages (from-to)17-22
Number of pages6
JournalNeuroscience Letters
Volume70
Issue number1
DOIs
StatePublished - Sep 25 1986

Keywords

  • Autoradiography
  • Brain
  • Feeding
  • Insulin
  • Rat
  • Receptor

ASJC Scopus subject areas

  • Neuroscience(all)

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