Localization of somatostatin mRNA in the gut, pancreas and thyroid gland of the rat using antisense RNA probes for in situ hybridization.

H. Höfler, H. Childers, M. R. Montminy, Richard Goodman, R. M. Lechan, R. A. DeLellis, A. S. Tischler, H. J. Wolfe

Research output: Contribution to journalArticle

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Abstract

A method for the localization of rat pre-prosomatostatin mRNA by in situ hybridization with 32P- and 3H-labeled antisense RNA probes is reported. Somatostatin mRNA was detected in endocrine cells of the rat gut mucosa, pancreas and thyroid in a distribution identical to immunoreactive somatostatin. In addition, in situ hybridization allowed localization of reactive neurons in the submucous and myenteric plexus of the gut, sites which are variably positive or negative for immunoreactive somatostatin even after colchicine treatment. These studies indicate that in situ hybridization is more sensitive than immunohistochemistry in some instances for demonstration of somatostatin in gene expression.

Original languageEnglish (US)
Pages (from-to)101-105
Number of pages5
JournalActa histochemica. Supplementband
Volume34
StatePublished - 1987
Externally publishedYes

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RNA Probes
Antisense RNA
Somatostatin
In Situ Hybridization
Pancreas
Thyroid Gland
Messenger RNA
Submucous Plexus
Enteroendocrine Cells
Myenteric Plexus
RNA Precursors
Colchicine
Mucous Membrane
Immunohistochemistry
Gene Expression
Neurons

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Localization of somatostatin mRNA in the gut, pancreas and thyroid gland of the rat using antisense RNA probes for in situ hybridization. / Höfler, H.; Childers, H.; Montminy, M. R.; Goodman, Richard; Lechan, R. M.; DeLellis, R. A.; Tischler, A. S.; Wolfe, H. J.

In: Acta histochemica. Supplementband, Vol. 34, 1987, p. 101-105.

Research output: Contribution to journalArticle

Höfler, H, Childers, H, Montminy, MR, Goodman, R, Lechan, RM, DeLellis, RA, Tischler, AS & Wolfe, HJ 1987, 'Localization of somatostatin mRNA in the gut, pancreas and thyroid gland of the rat using antisense RNA probes for in situ hybridization.', Acta histochemica. Supplementband, vol. 34, pp. 101-105.
Höfler, H. ; Childers, H. ; Montminy, M. R. ; Goodman, Richard ; Lechan, R. M. ; DeLellis, R. A. ; Tischler, A. S. ; Wolfe, H. J. / Localization of somatostatin mRNA in the gut, pancreas and thyroid gland of the rat using antisense RNA probes for in situ hybridization. In: Acta histochemica. Supplementband. 1987 ; Vol. 34. pp. 101-105.
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AU - Childers, H.

AU - Montminy, M. R.

AU - Goodman, Richard

AU - Lechan, R. M.

AU - DeLellis, R. A.

AU - Tischler, A. S.

AU - Wolfe, H. J.

PY - 1987

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