TY - JOUR
T1 - Localization of serotonin subtype 6 receptor messenger RNA in the rat brain by in situ hybridization histochemistry
AU - Ward, R. P.
AU - Hamblin, M. W.
AU - Lachowicz, J. E.
AU - Hoffman, B. J.
AU - Sibley, D. R.
AU - Dorsa, D. M.
N1 - Funding Information:
Acknowledgements--We are grateful to Anne Kalliomaki for her technical assistance. This work was supported by NIH grants NS20311 and GM07266, The Pharmaceutical Manufacturers Association Foundation, as well as by the Department of Veterans Affairs.
PY - 1995/2
Y1 - 1995/2
N2 - The serotonin receptor subtype 6, which raises intracellular cyclic AMP via stimulatory G-proteins, has recently been cloned and characterized. To determine the distribution of serotonin subtype 6 messenger RNA, in situ hybridization was performed in coronal sections of rat brain. 35S-labeled riboprobe, complementary to the 5′ non-coding region of the serotonin subtype 6 messenger RNA, and a 33P-labeled riboprobe complementary to its 3′ non-coding region, were used for hybridization. Serotonin subtype 6 receptor message was found in serotonin projection fields, rather than regions of serotonin-containing cell bodies, suggesting that the receptor is mainly postsynaptic. Hybridization signal was highest in olfactory tubercle, as well as prominent in the striatum, nucleus accumbens, dentate gyrus, and CA1, CA2 and CA3 of the hippocampus. Less intense hybridization was observed in cerebellum, some diencephalic nuclei, the amygdala, and layers 2, 3, 4 and 6 of the cortex. This pattern of hybridization was observed with both probes, but not when sense transcripts were used. Because the serotonin subtype 6 receptor has a high affinity for the atypical antipsychotic clozapine, and because striatum and nucleus accumbens are proposed sites of antipsychotic drug effects, the possibility is raised that this receptor may play an important role in mediating the effects of the atypical antipsychotic agents.
AB - The serotonin receptor subtype 6, which raises intracellular cyclic AMP via stimulatory G-proteins, has recently been cloned and characterized. To determine the distribution of serotonin subtype 6 messenger RNA, in situ hybridization was performed in coronal sections of rat brain. 35S-labeled riboprobe, complementary to the 5′ non-coding region of the serotonin subtype 6 messenger RNA, and a 33P-labeled riboprobe complementary to its 3′ non-coding region, were used for hybridization. Serotonin subtype 6 receptor message was found in serotonin projection fields, rather than regions of serotonin-containing cell bodies, suggesting that the receptor is mainly postsynaptic. Hybridization signal was highest in olfactory tubercle, as well as prominent in the striatum, nucleus accumbens, dentate gyrus, and CA1, CA2 and CA3 of the hippocampus. Less intense hybridization was observed in cerebellum, some diencephalic nuclei, the amygdala, and layers 2, 3, 4 and 6 of the cortex. This pattern of hybridization was observed with both probes, but not when sense transcripts were used. Because the serotonin subtype 6 receptor has a high affinity for the atypical antipsychotic clozapine, and because striatum and nucleus accumbens are proposed sites of antipsychotic drug effects, the possibility is raised that this receptor may play an important role in mediating the effects of the atypical antipsychotic agents.
UR - http://www.scopus.com/inward/record.url?scp=0028896011&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028896011&partnerID=8YFLogxK
U2 - 10.1016/0306-4522(94)00439-C
DO - 10.1016/0306-4522(94)00439-C
M3 - Article
C2 - 7753378
AN - SCOPUS:0028896011
SN - 0306-4522
VL - 64
SP - 1105
EP - 1111
JO - Neuroscience
JF - Neuroscience
IS - 4
ER -