Localization of proteasomes in human oocytes and preimplantation embryos

Cezary Wojcik, M. Benchaib, J. Lornage, J. C. Czyba, J. F. Guerin

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

In the present study we describe the localization of proteasomes in human oocytes, apoptotic preimplantation embryos, and triploid preimplantation embryos by means of immunolabelling with the MCP21 monoclonal antibody detected by confocal microscopy. While in the oocytes proteasomes are scattered throughout the cytoplasm, in the pronuclear zygote they appear to concentrate at the periphery of the cytoplasm and do not enter the pronuclei. During early cleavage stages, proteasome immunolabelling is concentrated in the nuclei, while the examination of triploid blastocysts showed that proteasomes had a similar cellular distribution to somatic cell lines, i.e. in the nuclei but not in the nucleoli or the cytoplasm. It appears that the distribution of proteasomes dramatically changes during human preimplantation embryo development.

Original languageEnglish (US)
Pages (from-to)331-336
Number of pages6
JournalMolecular Human Reproduction
Volume6
Issue number4
StatePublished - Apr 2000
Externally publishedYes

Fingerprint

Blastocyst
Proteasome Endopeptidase Complex
Oocytes
Triploidy
Cytoplasm
Zygote
Confocal Microscopy
Embryonic Development
Monoclonal Antibodies
Cell Line

Keywords

  • Development
  • Embryo
  • Immunolabelling
  • Oocyte
  • Proteasome

ASJC Scopus subject areas

  • Obstetrics and Gynecology
  • Genetics
  • Developmental Biology
  • Embryology
  • Cell Biology

Cite this

Wojcik, C., Benchaib, M., Lornage, J., Czyba, J. C., & Guerin, J. F. (2000). Localization of proteasomes in human oocytes and preimplantation embryos. Molecular Human Reproduction, 6(4), 331-336.

Localization of proteasomes in human oocytes and preimplantation embryos. / Wojcik, Cezary; Benchaib, M.; Lornage, J.; Czyba, J. C.; Guerin, J. F.

In: Molecular Human Reproduction, Vol. 6, No. 4, 04.2000, p. 331-336.

Research output: Contribution to journalArticle

Wojcik, C, Benchaib, M, Lornage, J, Czyba, JC & Guerin, JF 2000, 'Localization of proteasomes in human oocytes and preimplantation embryos', Molecular Human Reproduction, vol. 6, no. 4, pp. 331-336.
Wojcik C, Benchaib M, Lornage J, Czyba JC, Guerin JF. Localization of proteasomes in human oocytes and preimplantation embryos. Molecular Human Reproduction. 2000 Apr;6(4):331-336.
Wojcik, Cezary ; Benchaib, M. ; Lornage, J. ; Czyba, J. C. ; Guerin, J. F. / Localization of proteasomes in human oocytes and preimplantation embryos. In: Molecular Human Reproduction. 2000 ; Vol. 6, No. 4. pp. 331-336.
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