Localization of proteasomal antigens during different phases of the cell cycle in HeLa cells

Cezary Wojcik, N. Paweletz, D. Schroeter

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

We localized two different proteasome-associated epitopes with the use of two specific monoclonal antibodies, MCP21 and anti-p25, in interphase and mitotic HeLa cells cultured in vitro. We provide evidence for an association of those antigens with the spindle poles in metaphase and with the midbody region in anaphase and telophase. Triton-nonextractable structures are also labeled in interphase. Moreover, the labeling pattern of interphase cells obtained with the monoclonal antibodies MCP21 and anti-p25 differs considerably, and in the case of MCP21 displays clear cell cycle-specific changes: nuclear labeling is absent or very weak in G1 and S phases of the cell cycle, whereas it is strong in G2 phase. This might reflect the existence of different subpopulations of proteasomes in the cell.

Original languageEnglish (US)
Pages (from-to)191-198
Number of pages8
JournalEuropean Journal of Cell Biology
Volume68
Issue number2
StatePublished - 1995
Externally publishedYes

Fingerprint

Interphase
HeLa Cells
Cell Cycle
Proteasome Endopeptidase Complex
Antigens
Monoclonal Antibodies
Spindle Poles
Telophase
Anaphase
G2 Phase
G1 Phase
Metaphase
S Phase
Epitopes

Keywords

  • Cell cycle
  • HeLa cells
  • Mitosis
  • Prosomes
  • Proteasomes

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Localization of proteasomal antigens during different phases of the cell cycle in HeLa cells. / Wojcik, Cezary; Paweletz, N.; Schroeter, D.

In: European Journal of Cell Biology, Vol. 68, No. 2, 1995, p. 191-198.

Research output: Contribution to journalArticle

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