TY - JOUR
T1 - Localization of nyctalopin in the mammalian retina
AU - Morgans, Catherine W.
AU - Ren, Gaoying
AU - Akileswaran, Lakshmi
PY - 2006/3
Y1 - 2006/3
N2 - Complete X-linked congenital stationary night blindness (CSNB1) is a hereditary visual disease characterized by abnormalities in both the dark- and light-adapted electroretinogram, consistent with a defect in synaptic transmission between photoreceptors and ON-bipolar cells. The gene responsible for CSNB1, NYX, encodes a novel, leucine-rich repeat protein, nyctalopin. Consistent with its predicted glycosylphosphatidylinositol linkage, we show that recombinant nyctalopin is targeted to the extracellular cell surface in transfected HEK293 cells. Within the retina, strong nyctalopin immunoreactivity is present in the outer plexiform layer, the site of the photoreceptor to bipolar cell synapses. Double labelling of nyctalopin and known synaptic proteins in the outer plexiform layer indicate that nyctalopin is associated with the ribbon synapses of both rod and cone terminals. In the inner plexiform layer, nyctalopin immunoreactivity is associated with rod bipolar cell terminals. Our findings support a role for nyctalopin in synaptic transmission and/or synapse formation at ribbon synapses in the retina.
AB - Complete X-linked congenital stationary night blindness (CSNB1) is a hereditary visual disease characterized by abnormalities in both the dark- and light-adapted electroretinogram, consistent with a defect in synaptic transmission between photoreceptors and ON-bipolar cells. The gene responsible for CSNB1, NYX, encodes a novel, leucine-rich repeat protein, nyctalopin. Consistent with its predicted glycosylphosphatidylinositol linkage, we show that recombinant nyctalopin is targeted to the extracellular cell surface in transfected HEK293 cells. Within the retina, strong nyctalopin immunoreactivity is present in the outer plexiform layer, the site of the photoreceptor to bipolar cell synapses. Double labelling of nyctalopin and known synaptic proteins in the outer plexiform layer indicate that nyctalopin is associated with the ribbon synapses of both rod and cone terminals. In the inner plexiform layer, nyctalopin immunoreactivity is associated with rod bipolar cell terminals. Our findings support a role for nyctalopin in synaptic transmission and/or synapse formation at ribbon synapses in the retina.
KW - Bipolar cell
KW - CSNB1
KW - Photoreceptor
KW - Ribbon synapse
KW - mGluR6
UR - http://www.scopus.com/inward/record.url?scp=33645098679&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33645098679&partnerID=8YFLogxK
U2 - 10.1111/j.1460-9568.2006.04647.x
DO - 10.1111/j.1460-9568.2006.04647.x
M3 - Article
C2 - 16553780
AN - SCOPUS:33645098679
SN - 0953-816X
VL - 23
SP - 1163
EP - 1171
JO - European Journal of Neuroscience
JF - European Journal of Neuroscience
IS - 5
ER -