Localization of nyctalopin in the mammalian retina

Catherine Morgans, Gaoying Ren, Lakshmi Akileswaran

    Research output: Contribution to journalArticle

    53 Citations (Scopus)

    Abstract

    Complete X-linked congenital stationary night blindness (CSNB1) is a hereditary visual disease characterized by abnormalities in both the dark- and light-adapted electroretinogram, consistent with a defect in synaptic transmission between photoreceptors and ON-bipolar cells. The gene responsible for CSNB1, NYX, encodes a novel, leucine-rich repeat protein, nyctalopin. Consistent with its predicted glycosylphosphatidylinositol linkage, we show that recombinant nyctalopin is targeted to the extracellular cell surface in transfected HEK293 cells. Within the retina, strong nyctalopin immunoreactivity is present in the outer plexiform layer, the site of the photoreceptor to bipolar cell synapses. Double labelling of nyctalopin and known synaptic proteins in the outer plexiform layer indicate that nyctalopin is associated with the ribbon synapses of both rod and cone terminals. In the inner plexiform layer, nyctalopin immunoreactivity is associated with rod bipolar cell terminals. Our findings support a role for nyctalopin in synaptic transmission and/or synapse formation at ribbon synapses in the retina.

    Original languageEnglish (US)
    Pages (from-to)1163-1171
    Number of pages9
    JournalEuropean Journal of Neuroscience
    Volume23
    Issue number5
    DOIs
    StatePublished - Mar 2006

    Fingerprint

    Synapses
    Retina
    Synaptic Transmission
    Glycosylphosphatidylinositols
    Vertebrate Photoreceptor Cells
    Inborn Genetic Diseases
    HEK293 Cells
    Light
    Genes
    Proteins

    Keywords

    • Bipolar cell
    • CSNB1
    • mGluR6
    • Photoreceptor
    • Ribbon synapse

    ASJC Scopus subject areas

    • Neuroscience(all)

    Cite this

    Localization of nyctalopin in the mammalian retina. / Morgans, Catherine; Ren, Gaoying; Akileswaran, Lakshmi.

    In: European Journal of Neuroscience, Vol. 23, No. 5, 03.2006, p. 1163-1171.

    Research output: Contribution to journalArticle

    Morgans, Catherine ; Ren, Gaoying ; Akileswaran, Lakshmi. / Localization of nyctalopin in the mammalian retina. In: European Journal of Neuroscience. 2006 ; Vol. 23, No. 5. pp. 1163-1171.
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