Localization of interleukin-1α, type 1 interleukin-1 receptor and interleukin-1 receptor antagonist in the synovial membrane and cartilage/pannus junction in rheumatoid arthritis

B. W. Deleuran, Cong-Qiu Chu, M. Field, F. M. Brennan, P. Katsikis, M. Feldmann, R. N. Maini

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Abstract

Using monoclonal antibodies and immunohistochemical techniques we have investigated the presence and distribution of interleukin-1α (IL-1α), type 1 IL-1 receptor (IL-1R1) and of interleukin-1 receptor antagonist (IL-1ra) in synovial tissue from 18 rheumatoid arthritis (RA) and eight osteoarthritis (OA) patients and in eight normal synovial tissue samples. IL-1α and IL-1R1 were found in all of the samples examined. In RA, there were a large number of synovial cells expressing IL-lα and IL-1Rl, with 85 and 90% positive cells in the lining layer, 45 and.80% in the interaggregate area, and 90% of the vascular endothelial cells. In the lymphoid aggregates, 20% of the cells contained IL-lα and 70% expressed IL-1R1. IL-1α and IL-1R1 expressing cells showed a similar distribution in OA synovial membrane, but there was a smaller number of positive cells in the deeper area; and the staining intensity was lower. In contrast to IL-1α and IL-1R1, IL-1ra was found only in 10/18 RA, 5/8 OA and 2/8 normal tissue samples. IL-1ra was detected in 35% of RA and 45% OA lining layer cells; 25% RA and 35% OA vascular endothelium; 10% RA and 15% OA interstitial cells and 30% cells in RA lymphoid aggregate. The staining intensity in both RA and OA tissues was comparably low. The presence of IL-1ra in RA and OA tissues was confirmed by Northern blot analysis for IL-1ra mRNA. At the RA cartilage/pannus junction, up to 90% of the pannus cells expressed IL-1α and IL-1R1, whereas IL-1ra was present in less than 10% of the cells. Some chondrocytes also expressed IL-1α and IL-1R1. In the normal cartilage/synovium junction fewer cells expressed IL-1α, IL-1R1 and IL-1ra. These results provide histological evidence that IL-1α, IL-1R1 and IL-1ra are present in the inflamed synovium, indicating that the IL-1 pathway is expressed and regulated within diseased joints.

Original languageEnglish (US)
Pages (from-to)801-809
Number of pages9
JournalRheumatology
Volume31
Issue number12
DOIs
StatePublished - Dec 1992
Externally publishedYes

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Interleukin-1 Type I Receptors
interleukins
Cartilage
Interleukin
arthritis
cartilage
Rheumatoid Arthritis
Interleukin-1 Receptors
Synovial Membrane
Interleukin-1
Receptor
Membrane
Osteoarthritis
Tissue
membranes
Membranes
Cells
Linings
Cell
Monoclonal antibodies

Keywords

  • Interleukin-1 receptor
  • Interleukin-1 receptor antagonist
  • Interleukin-1α
  • Rheumatoid arthritis
  • Synovial tissue

ASJC Scopus subject areas

  • Molecular Biology
  • Statistics and Probability
  • Computational Mathematics
  • Condensed Matter Physics
  • Applied Mathematics
  • Pharmacology (medical)
  • Rheumatology
  • Neuroscience(all)

Cite this

Localization of interleukin-1α, type 1 interleukin-1 receptor and interleukin-1 receptor antagonist in the synovial membrane and cartilage/pannus junction in rheumatoid arthritis. / Deleuran, B. W.; Chu, Cong-Qiu; Field, M.; Brennan, F. M.; Katsikis, P.; Feldmann, M.; Maini, R. N.

In: Rheumatology, Vol. 31, No. 12, 12.1992, p. 801-809.

Research output: Contribution to journalArticle

Deleuran, B. W. ; Chu, Cong-Qiu ; Field, M. ; Brennan, F. M. ; Katsikis, P. ; Feldmann, M. ; Maini, R. N. / Localization of interleukin-1α, type 1 interleukin-1 receptor and interleukin-1 receptor antagonist in the synovial membrane and cartilage/pannus junction in rheumatoid arthritis. In: Rheumatology. 1992 ; Vol. 31, No. 12. pp. 801-809.
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abstract = "Using monoclonal antibodies and immunohistochemical techniques we have investigated the presence and distribution of interleukin-1α (IL-1α), type 1 IL-1 receptor (IL-1R1) and of interleukin-1 receptor antagonist (IL-1ra) in synovial tissue from 18 rheumatoid arthritis (RA) and eight osteoarthritis (OA) patients and in eight normal synovial tissue samples. IL-1α and IL-1R1 were found in all of the samples examined. In RA, there were a large number of synovial cells expressing IL-lα and IL-1Rl, with 85 and 90{\%} positive cells in the lining layer, 45 and.80{\%} in the interaggregate area, and 90{\%} of the vascular endothelial cells. In the lymphoid aggregates, 20{\%} of the cells contained IL-lα and 70{\%} expressed IL-1R1. IL-1α and IL-1R1 expressing cells showed a similar distribution in OA synovial membrane, but there was a smaller number of positive cells in the deeper area; and the staining intensity was lower. In contrast to IL-1α and IL-1R1, IL-1ra was found only in 10/18 RA, 5/8 OA and 2/8 normal tissue samples. IL-1ra was detected in 35{\%} of RA and 45{\%} OA lining layer cells; 25{\%} RA and 35{\%} OA vascular endothelium; 10{\%} RA and 15{\%} OA interstitial cells and 30{\%} cells in RA lymphoid aggregate. The staining intensity in both RA and OA tissues was comparably low. The presence of IL-1ra in RA and OA tissues was confirmed by Northern blot analysis for IL-1ra mRNA. At the RA cartilage/pannus junction, up to 90{\%} of the pannus cells expressed IL-1α and IL-1R1, whereas IL-1ra was present in less than 10{\%} of the cells. Some chondrocytes also expressed IL-1α and IL-1R1. In the normal cartilage/synovium junction fewer cells expressed IL-1α, IL-1R1 and IL-1ra. These results provide histological evidence that IL-1α, IL-1R1 and IL-1ra are present in the inflamed synovium, indicating that the IL-1 pathway is expressed and regulated within diseased joints.",
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N2 - Using monoclonal antibodies and immunohistochemical techniques we have investigated the presence and distribution of interleukin-1α (IL-1α), type 1 IL-1 receptor (IL-1R1) and of interleukin-1 receptor antagonist (IL-1ra) in synovial tissue from 18 rheumatoid arthritis (RA) and eight osteoarthritis (OA) patients and in eight normal synovial tissue samples. IL-1α and IL-1R1 were found in all of the samples examined. In RA, there were a large number of synovial cells expressing IL-lα and IL-1Rl, with 85 and 90% positive cells in the lining layer, 45 and.80% in the interaggregate area, and 90% of the vascular endothelial cells. In the lymphoid aggregates, 20% of the cells contained IL-lα and 70% expressed IL-1R1. IL-1α and IL-1R1 expressing cells showed a similar distribution in OA synovial membrane, but there was a smaller number of positive cells in the deeper area; and the staining intensity was lower. In contrast to IL-1α and IL-1R1, IL-1ra was found only in 10/18 RA, 5/8 OA and 2/8 normal tissue samples. IL-1ra was detected in 35% of RA and 45% OA lining layer cells; 25% RA and 35% OA vascular endothelium; 10% RA and 15% OA interstitial cells and 30% cells in RA lymphoid aggregate. The staining intensity in both RA and OA tissues was comparably low. The presence of IL-1ra in RA and OA tissues was confirmed by Northern blot analysis for IL-1ra mRNA. At the RA cartilage/pannus junction, up to 90% of the pannus cells expressed IL-1α and IL-1R1, whereas IL-1ra was present in less than 10% of the cells. Some chondrocytes also expressed IL-1α and IL-1R1. In the normal cartilage/synovium junction fewer cells expressed IL-1α, IL-1R1 and IL-1ra. These results provide histological evidence that IL-1α, IL-1R1 and IL-1ra are present in the inflamed synovium, indicating that the IL-1 pathway is expressed and regulated within diseased joints.

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