Localization of cytomegalovirus proteins and genome during fulminant central nervous system infection in an ADIS patient

C. A. Wiley, R. D. Schrier, F. J. Denaro, Jay Nelson, P. W. Lampert, M. B. Oldstone

Research output: Contribution to journalArticle

107 Citations (Scopus)

Abstract

Approximately one-half of autopsied acquired immune deficiency syndrome (AIDS) patients demonstrate probable human cytomegalovirus (CMV) infection of the central nervous system (CNS). Because CMV in brain tissue or cerebrospinal fluid is difficult to culture, we used antisera, and radioactive probes to diagnose CMV infection in the brain of an autopsied AIDS patient, who died of a fulminant CNS and systemic infection with CMV, suggesting a complete seeding of the ependymal regions possibly followed by a uniform ventriculofugal spread of the virus deep into the parenchyma. Cytomegalic cells were observed in optic nerve, retina, ependymal and subependymal regions of the brain and in the motor (but not sensory) root-CNS junctions. Immunocytochemistry demonstrated viral antigen predominantly in cytomegalic cells, which also stained positively for glial fibrillary acidic protein, S-100, or neuron-specific enolase, but not a common leukocyte antigen. Virions were visible in these cells examined by electron microscopy. No viral replication was observed in pineocytes, pituicytes or the choroid plexus. Morphologically normal cells that were CMV antigen-negative proved to be infected after in situ hybridization with well-defined human CMV DNA fragments. Hence, morphologically normal glia and neurons show restricted replication of CMV, indicating that such cells may be latently infected.

Original languageEnglish (US)
Pages (from-to)127-139
Number of pages13
JournalJournal of Neuropathology and Experimental Neurology
Volume45
Issue number2
StatePublished - 1986
Externally publishedYes

Fingerprint

Central Nervous System Infections
Cytomegalovirus
Genome
Cytomegalovirus Infections
Proteins
Brain
Acquired Immunodeficiency Syndrome
Central Nervous System
CD45 Antigens
Choroid Plexus
Phosphopyruvate Hydratase
Viral Antigens
Glial Fibrillary Acidic Protein
Protein S
Optic Nerve
Neuroglia
Virion
In Situ Hybridization
Cerebrospinal Fluid
Retina

Keywords

  • Acquired immune deficiency syndrome
  • Cytomegalovirus
  • Encephalitis
  • Hybridization
  • Immunocytochemistry
  • In situ

ASJC Scopus subject areas

  • Clinical Neurology
  • Pathology and Forensic Medicine
  • Cellular and Molecular Neuroscience
  • Neurology
  • Neuroscience(all)

Cite this

Localization of cytomegalovirus proteins and genome during fulminant central nervous system infection in an ADIS patient. / Wiley, C. A.; Schrier, R. D.; Denaro, F. J.; Nelson, Jay; Lampert, P. W.; Oldstone, M. B.

In: Journal of Neuropathology and Experimental Neurology, Vol. 45, No. 2, 1986, p. 127-139.

Research output: Contribution to journalArticle

Wiley, C. A. ; Schrier, R. D. ; Denaro, F. J. ; Nelson, Jay ; Lampert, P. W. ; Oldstone, M. B. / Localization of cytomegalovirus proteins and genome during fulminant central nervous system infection in an ADIS patient. In: Journal of Neuropathology and Experimental Neurology. 1986 ; Vol. 45, No. 2. pp. 127-139.
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