Localization of androgen receptor in the follicle and corpus luteum of the primate ovary during the menstrual cycle

S. Hild-Petito, N. B. West, R. M. Brenner, Richard Stouffer

    Research output: Contribution to journalArticle

    92 Citations (Scopus)

    Abstract

    Ovarian androgens may act locally to modulate follicular and luteal function in various species. This study examined the distribution of androgen receptors within the primate ovary throughout the menstrual cycle. Ovaries were collected from rhesus and cynomolgus monkeys during the early, mid-, and late (n = 3-5 per stage) follicular and luteal phases of the cycle. The tissues were processed for indirect immunocytochemical localization of androgen receptors with a specific monoclonal antibody against human androgen receptor (AN1-15). In addition, ovaries (n = 3) were collected from rhesus monkeys for biochemical detection of androgen receptor using 3H-androgen and AN1-15. Specific immunocytochemical staining, as determined by comparing adjacent tissue sections incubated with either AN1-15 or a nonspecific control antibody, was exclusively nuclear. Androgen receptor was detected in the germinal epithelium and ovarian stroma at all stages of the cycle. The thecal and granulosa cells of growing follicles, and of many but not all atretic follicles, contained androgen receptors. Luteinizing granulosa cells of the periovulatory follicle and luteal cells from the early and midluteal phase stained intensely for androgen receptor. Regressing corpora lutea of the late luteal phase also stained for androgen receptor; however, fully regressed corpora lutea in the early follicular phase of the next cycle did not exhibit receptor staining. Luteal cells that were androgen receptor-positive also stained histochemically for the presence of 3β-hydroxysteroid dehydrogenase. Sucrose gradient analysis with radiolabeled androgen demonstrated a shift in the androgen receptor peak in monkey ovarian tissue upon addition of AN1-15, confirming the presence of androgen receptor. These data support a receptor-mediated autocrine or paracrine role for androgen in the theca and granulosa layers during follicular development in the primate ovary. Moreover, the evidence suggests for the first time a local androgen action within the corpus luteum during the menstrual cycle.

    Original languageEnglish (US)
    Pages (from-to)561-568
    Number of pages8
    JournalBiology of Reproduction
    Volume44
    Issue number3
    StatePublished - 1991

    Fingerprint

    Corpus Luteum
    Androgen Receptors
    Menstrual Cycle
    Primates
    Ovary
    Androgens
    Luteal Cells
    Follicular Phase
    Granulosa Cells
    Luteal Phase
    Macaca mulatta
    3-Hydroxysteroid Dehydrogenases
    Staining and Labeling
    Ovarian Follicle
    Macaca fascicularis
    Haplorhini
    Sucrose
    Epithelium
    Monoclonal Antibodies
    Antibodies

    ASJC Scopus subject areas

    • Cell Biology
    • Developmental Biology
    • Embryology

    Cite this

    Localization of androgen receptor in the follicle and corpus luteum of the primate ovary during the menstrual cycle. / Hild-Petito, S.; West, N. B.; Brenner, R. M.; Stouffer, Richard.

    In: Biology of Reproduction, Vol. 44, No. 3, 1991, p. 561-568.

    Research output: Contribution to journalArticle

    @article{57ab2cda5341444f90be2e4480c128c2,
    title = "Localization of androgen receptor in the follicle and corpus luteum of the primate ovary during the menstrual cycle",
    abstract = "Ovarian androgens may act locally to modulate follicular and luteal function in various species. This study examined the distribution of androgen receptors within the primate ovary throughout the menstrual cycle. Ovaries were collected from rhesus and cynomolgus monkeys during the early, mid-, and late (n = 3-5 per stage) follicular and luteal phases of the cycle. The tissues were processed for indirect immunocytochemical localization of androgen receptors with a specific monoclonal antibody against human androgen receptor (AN1-15). In addition, ovaries (n = 3) were collected from rhesus monkeys for biochemical detection of androgen receptor using 3H-androgen and AN1-15. Specific immunocytochemical staining, as determined by comparing adjacent tissue sections incubated with either AN1-15 or a nonspecific control antibody, was exclusively nuclear. Androgen receptor was detected in the germinal epithelium and ovarian stroma at all stages of the cycle. The thecal and granulosa cells of growing follicles, and of many but not all atretic follicles, contained androgen receptors. Luteinizing granulosa cells of the periovulatory follicle and luteal cells from the early and midluteal phase stained intensely for androgen receptor. Regressing corpora lutea of the late luteal phase also stained for androgen receptor; however, fully regressed corpora lutea in the early follicular phase of the next cycle did not exhibit receptor staining. Luteal cells that were androgen receptor-positive also stained histochemically for the presence of 3β-hydroxysteroid dehydrogenase. Sucrose gradient analysis with radiolabeled androgen demonstrated a shift in the androgen receptor peak in monkey ovarian tissue upon addition of AN1-15, confirming the presence of androgen receptor. These data support a receptor-mediated autocrine or paracrine role for androgen in the theca and granulosa layers during follicular development in the primate ovary. Moreover, the evidence suggests for the first time a local androgen action within the corpus luteum during the menstrual cycle.",
    author = "S. Hild-Petito and West, {N. B.} and Brenner, {R. M.} and Richard Stouffer",
    year = "1991",
    language = "English (US)",
    volume = "44",
    pages = "561--568",
    journal = "Biology of Reproduction",
    issn = "0006-3363",
    publisher = "Society for the Study of Reproduction",
    number = "3",

    }

    TY - JOUR

    T1 - Localization of androgen receptor in the follicle and corpus luteum of the primate ovary during the menstrual cycle

    AU - Hild-Petito, S.

    AU - West, N. B.

    AU - Brenner, R. M.

    AU - Stouffer, Richard

    PY - 1991

    Y1 - 1991

    N2 - Ovarian androgens may act locally to modulate follicular and luteal function in various species. This study examined the distribution of androgen receptors within the primate ovary throughout the menstrual cycle. Ovaries were collected from rhesus and cynomolgus monkeys during the early, mid-, and late (n = 3-5 per stage) follicular and luteal phases of the cycle. The tissues were processed for indirect immunocytochemical localization of androgen receptors with a specific monoclonal antibody against human androgen receptor (AN1-15). In addition, ovaries (n = 3) were collected from rhesus monkeys for biochemical detection of androgen receptor using 3H-androgen and AN1-15. Specific immunocytochemical staining, as determined by comparing adjacent tissue sections incubated with either AN1-15 or a nonspecific control antibody, was exclusively nuclear. Androgen receptor was detected in the germinal epithelium and ovarian stroma at all stages of the cycle. The thecal and granulosa cells of growing follicles, and of many but not all atretic follicles, contained androgen receptors. Luteinizing granulosa cells of the periovulatory follicle and luteal cells from the early and midluteal phase stained intensely for androgen receptor. Regressing corpora lutea of the late luteal phase also stained for androgen receptor; however, fully regressed corpora lutea in the early follicular phase of the next cycle did not exhibit receptor staining. Luteal cells that were androgen receptor-positive also stained histochemically for the presence of 3β-hydroxysteroid dehydrogenase. Sucrose gradient analysis with radiolabeled androgen demonstrated a shift in the androgen receptor peak in monkey ovarian tissue upon addition of AN1-15, confirming the presence of androgen receptor. These data support a receptor-mediated autocrine or paracrine role for androgen in the theca and granulosa layers during follicular development in the primate ovary. Moreover, the evidence suggests for the first time a local androgen action within the corpus luteum during the menstrual cycle.

    AB - Ovarian androgens may act locally to modulate follicular and luteal function in various species. This study examined the distribution of androgen receptors within the primate ovary throughout the menstrual cycle. Ovaries were collected from rhesus and cynomolgus monkeys during the early, mid-, and late (n = 3-5 per stage) follicular and luteal phases of the cycle. The tissues were processed for indirect immunocytochemical localization of androgen receptors with a specific monoclonal antibody against human androgen receptor (AN1-15). In addition, ovaries (n = 3) were collected from rhesus monkeys for biochemical detection of androgen receptor using 3H-androgen and AN1-15. Specific immunocytochemical staining, as determined by comparing adjacent tissue sections incubated with either AN1-15 or a nonspecific control antibody, was exclusively nuclear. Androgen receptor was detected in the germinal epithelium and ovarian stroma at all stages of the cycle. The thecal and granulosa cells of growing follicles, and of many but not all atretic follicles, contained androgen receptors. Luteinizing granulosa cells of the periovulatory follicle and luteal cells from the early and midluteal phase stained intensely for androgen receptor. Regressing corpora lutea of the late luteal phase also stained for androgen receptor; however, fully regressed corpora lutea in the early follicular phase of the next cycle did not exhibit receptor staining. Luteal cells that were androgen receptor-positive also stained histochemically for the presence of 3β-hydroxysteroid dehydrogenase. Sucrose gradient analysis with radiolabeled androgen demonstrated a shift in the androgen receptor peak in monkey ovarian tissue upon addition of AN1-15, confirming the presence of androgen receptor. These data support a receptor-mediated autocrine or paracrine role for androgen in the theca and granulosa layers during follicular development in the primate ovary. Moreover, the evidence suggests for the first time a local androgen action within the corpus luteum during the menstrual cycle.

    UR - http://www.scopus.com/inward/record.url?scp=0025974241&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=0025974241&partnerID=8YFLogxK

    M3 - Article

    C2 - 2015374

    AN - SCOPUS:0025974241

    VL - 44

    SP - 561

    EP - 568

    JO - Biology of Reproduction

    JF - Biology of Reproduction

    SN - 0006-3363

    IS - 3

    ER -