Localization of a proteasonal antigen in human spermatozoa: Immunohistochemical electron microscopic study

L. P. Bialy, H. T. Ziemba, P. Marianowski, S. Fraçki, M. Bury, C. Wójcik

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

The ultrastructural localization of a proteasonal antigen in human spermatozoa was studied by means of immunolabeling with the MPC21 monoclonal antibody and secondary gold labeled antibody with 1.4 nm gold particles in combination with silver enhancement reaction using pre-embedding technique. The labeling was found in the acrosomal and postacrosomal regions, in the connecting-piece (neck) and, in some cases, in the middle-piece and also in the residual bodies. There was no significant reaction in condensed chromatin. In some abnormal forms of spermatozoa, in which the chromatin was not well condensed, the labeling in nuclei was present. The nuclear vacuoles with looser chromatin were usually strongly labeled. The nuclei of cells representing different stages of spermatogenesis, that were present in semen samples, were also labeled.

Original languageEnglish (US)
Pages (from-to)129-130
Number of pages2
JournalFolia Histochemica et Cytobiologica
Volume39
Issue number2
StatePublished - Jan 1 2001

Keywords

  • Electron
  • Immunolabelling
  • Proteasome
  • Spermatozoa

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology

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