Localization and structural characterization of insulin-like growth factor receptors in mammalian retina

I. Ocrant, K. L. Valentino, M. G. King, T. H. Wimpy, Ronald (Ron) Rosenfeld, D. G. Baskin

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Insulin-like growth factors (IGFs) are peptide mitogens, structurally related to insulin, whose biological actions in the CNS are incompletely known. The retina is largely uncharacterized with respect to IGF receptors. We, therefore, studied IGF receptors in bovine and murine retinal tissues by immunohistochemistry, autoradiographic localization, and affinity labeling. Notable IGF-II receptor immunoreactivity was found in retinal pigment epithelium (RPE), with intermediate levels in choroid, low levels in the inner and outer plexiform layers and outer nuclear layer, and very low levels in other regions. Autoradiographic localization using [125I]IGF-II confirmed the IGF-II receptor immunohistochemistry. Autoradiographic localization using [125I]IGF-I labeled the nuclear layers and the photoreceptor regions. Affinity labeling disclosed differences in the apparent mol wt of IGF-I and IGF-II receptors from bovine eye tissues and those from liver and brain. IGF-I receptor α-subunits (the IGF-binding subunit) migrated at: liver, 139,000; brain, 125,000; RPE, 125,000 and 135,000 (two sizes); and retina, 125,000 and 135,000. IGF-II receptors migrated at: liver, 245,000; brain, 235,000; RPE, 240,000; and retina, 230,000. We conclude that mammalian retina contains both IGF-I and -II receptors, which differ from those found in other tissues and have a characteristic spatial distribution within the retina.

Original languageEnglish (US)
Pages (from-to)2407-2413
Number of pages7
JournalEndocrinology
Volume125
Issue number5
StatePublished - 1989
Externally publishedYes

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IGF Type 2 Receptor
Somatomedin Receptors
Retina
Retinal Pigment Epithelium
IGF Type 1 Receptor
Somatomedins
Insulin-Like Growth Factor I
Liver
Brain
Immunohistochemistry
Insulin-Like Growth Factor II
Choroid
Mitogens
Insulin
Peptides

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Ocrant, I., Valentino, K. L., King, M. G., Wimpy, T. H., Rosenfeld, R. R., & Baskin, D. G. (1989). Localization and structural characterization of insulin-like growth factor receptors in mammalian retina. Endocrinology, 125(5), 2407-2413.

Localization and structural characterization of insulin-like growth factor receptors in mammalian retina. / Ocrant, I.; Valentino, K. L.; King, M. G.; Wimpy, T. H.; Rosenfeld, Ronald (Ron); Baskin, D. G.

In: Endocrinology, Vol. 125, No. 5, 1989, p. 2407-2413.

Research output: Contribution to journalArticle

Ocrant, I, Valentino, KL, King, MG, Wimpy, TH, Rosenfeld, RR & Baskin, DG 1989, 'Localization and structural characterization of insulin-like growth factor receptors in mammalian retina', Endocrinology, vol. 125, no. 5, pp. 2407-2413.
Ocrant I, Valentino KL, King MG, Wimpy TH, Rosenfeld RR, Baskin DG. Localization and structural characterization of insulin-like growth factor receptors in mammalian retina. Endocrinology. 1989;125(5):2407-2413.
Ocrant, I. ; Valentino, K. L. ; King, M. G. ; Wimpy, T. H. ; Rosenfeld, Ronald (Ron) ; Baskin, D. G. / Localization and structural characterization of insulin-like growth factor receptors in mammalian retina. In: Endocrinology. 1989 ; Vol. 125, No. 5. pp. 2407-2413.
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