Localization and retention in vitro of fluorescently labeled aortic baroreceptor terminals on neurons from the nucleus tractus solitarius

David Mendelowitz, Mingyong Yang, Michael C. Andresen, Diana L. Kunze

Research output: Contribution to journalArticle

85 Scopus citations

Abstract

The anterograde fluorescent tracer DiA was used to visualize baroreceptor fibers and synaptic terminals both in living and fixed tissue. Baroreceptor fibers labeled with DiA terminated as a dense synaptic field in the medial nucleus tractus solitarius (NTS), making synaptic contact on the soma, as well as processes of neurons that they innervated. A similar distribution and morphology was observed in baroreceptor fibers and terminals labeled with horseradish peroxidase. DiA also identified baroreceptor terminals and the neurons receiving these synaptic contacts in vitro. NTS neurons were dissociated from their surrounding tissue and identified by attached baroreceptor terminals that retained the fluorescent dye. These results will enable us to study the electrophysiological properties of dispersed neurons that receive identified baroreceptor synaptic terminals.

Original languageEnglish (US)
Pages (from-to)339-343
Number of pages5
JournalBrain research
Volume581
Issue number2
DOIs
StatePublished - May 29 1992

Keywords

  • Anterograde
  • Baroreflex
  • Cardiovascular
  • DiA
  • Dissociated
  • Horseradish peroxidase
  • Solitary tract
  • Tracer

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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