Localization and expression of GABA transporters in the suprachiasmatic nucleus

Michael Moldavan, Olga Cravetchi, Melissa Williams, Robert P. Irwin, Sue A. Aicher, Charles N. Allen

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

GABA is a principal neurotransmitter in the suprachiasmatic hypothalamic nucleus (SCN), the master circadian clock. Despite the importance of GABA and GABA uptake for functioning of the circadian pacemaker, the localization and expression of GABA transporters (GATs) in the SCN has not been investigated. The present studies used Western blot analysis, immunohistochemistry and electron microscopy to demonstrate the presence of GABA transporter 1 (GAT1) and GAT3 in the SCN. By using light microscopy, GAT1 and GAT3 were co-localized throughout the SCN, but were not expressed in the perikarya of arginine vasopressin- or vasoactive intestinal peptide-immunoreactive (-ir) neurons of adult rats, nor in the neuronal processes labelled with the neurofilament heavy chain. Using electron microscopy, GAT1- and GAT3-ir was found in glial processes surrounding unlabelled neuronal perikarya, axons, dendrites, and enveloped symmetric and asymmetric axo-dendritic synapses. Glial fibrillary acidic protein-ir astrocytes grown in cell culture were immunopositive for GAT1 and GAT3 and both GATs could be observed in the same glial cell. These data demonstrate that synapses in the SCN function as 'tripartite' synapses consisting of presynaptic axon terminals, postsynaptic membranes and astrocytes that contain GABA transporters. This model suggests that astrocytes expressing both GATs may regulate the extracellular GABA, and thereby modulate the activity of neuronal networks in the SCN. GABA transporters (GAT) are an important component of GABAergic neurotransmission in the suprachiasmatic nucleus (SCN), the master circadian clock. GAT1 and GAT3 were co-localized throughout the SCN but not in the perikarya of vasopressin or vasoactive intestinal peptide neurons. By electron microscopy, GAT1 and GAT3 were found in glial processes surrounding unlabeled neurons, axons, dendrites, and enveloped axodendritic synapses. GABAergic synapses in the SCN function as 'tripartite' synapses consisting of presynaptic axon terminals, postsynaptic GABA(A) receptors, and glial cells that contain GABA transporters. This model suggests that glial cells modulate SCN neuronal network activity by regulating the extracellular GABA concentration.

Original languageEnglish (US)
Pages (from-to)3018-3032
Number of pages15
JournalEuropean Journal of Neuroscience
Volume42
Issue number12
DOIs
StatePublished - Dec 1 2015

Keywords

  • Circadian rhythm
  • Electron microscopic imaging
  • Hypothalamus
  • Immunohistochemistry
  • Suprachiasmatic nucleus
  • Western blot

ASJC Scopus subject areas

  • Neuroscience(all)

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