Local Ca2+ release from internal stores controls exocytosis in pituitary gonadotrophs

F. W. Tse, A. Tse, B. Hille, H. Horstmann, Wolfhard Almers

Research output: Contribution to journalArticle

166 Citations (Scopus)

Abstract

Exocytosis and the cell-averaged cytosolic [Ca2+], [Ca2+](i), were tracked in single gonadotrophs. Cells released 100 granules/s at 1 μM [Ca2+](i) when gonadotropin-releasing hormone (GnRH) activated IP3- mediated Ca2+ release from internal stores, but only 1 granule/s when [Ca2+](i) was raised uniformly to 1 μM by other means. Strong exocytosis was then seen only at higher [Ca2+], (half-maximal at 16 μM). Parallel second messengers did not contribute to GaRH-induced exocytosis, because IP3 alone was as effective as GnRH, and because even GnRH failed to trigger rapid exocytosis when the [Ca2+](i) rise was blunted by EGTA. When [Ca2+](i) was released from stores, exocytosis depended on [Ca2+](i) rising rapidly, as if governed by Ca2+ flux into the cytosol. We suggest that IP3 releases Ca2+ selectively from subsurface cisternae, raising [Ca2+] near exocytic sites 5-fold above the cell average.

Original languageEnglish (US)
Pages (from-to)121-132
Number of pages12
JournalNeuron
Volume18
Issue number1
DOIs
StatePublished - Jan 1997
Externally publishedYes

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Gonadotrophs
Exocytosis
Gonadotropin-Releasing Hormone
Egtazic Acid
Second Messenger Systems
Cytosol

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Local Ca2+ release from internal stores controls exocytosis in pituitary gonadotrophs. / Tse, F. W.; Tse, A.; Hille, B.; Horstmann, H.; Almers, Wolfhard.

In: Neuron, Vol. 18, No. 1, 01.1997, p. 121-132.

Research output: Contribution to journalArticle

Tse, FW, Tse, A, Hille, B, Horstmann, H & Almers, W 1997, 'Local Ca2+ release from internal stores controls exocytosis in pituitary gonadotrophs', Neuron, vol. 18, no. 1, pp. 121-132. https://doi.org/10.1016/S0896-6273(01)80051-9
Tse, F. W. ; Tse, A. ; Hille, B. ; Horstmann, H. ; Almers, Wolfhard. / Local Ca2+ release from internal stores controls exocytosis in pituitary gonadotrophs. In: Neuron. 1997 ; Vol. 18, No. 1. pp. 121-132.
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