Local antigen in nonlymphoid tissue promotes resident memory CD8+ T cell formation during viral infection

Tahsin N. Khan, Jana L. Mooster, Augustus M. Kilgore, Jossef F. Osborn, Jeffrey C. Nolz

Research output: Research - peer-reviewArticle

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Abstract

Tissue-resident memory (Trm) CD8+ T cells are functionally distinct from their circulating counterparts and are potent mediators of host protection against reinfection. Whether local recognition of antigen in nonlymphoid tissues during infection can impact the formation of Trm populations remains unresolved. Using skin infections with vaccinia virus (VacV)-expressing model antigens, we found that local antigen recognition had a profound impact on Trm formation. Activated CD8+ T cells trafficked to VacV-infected skin in an inflammation-dependent, but antigen-independent, manner. However, after viral clearance, there was a subsequent ~50-fold increase in Trm formation when antigen was present in the tissue microenvironment. Secondary antigen stimulation in nonlymphoid tissue caused CD8+ T cells to rapidly express CD69 and be retained at the site of infection. Finally, Trm CD8+ T cells that formed during VacV infection in an antigen-dependent manner became potent stimulators of localized antigen-specific inflammatory responses in the skin. Thus, our studies indicate that the presence of antigen in the nonlymphoid tissue microenvironment plays a critical role in the formation of functional Trm CD8+ T cell populations, a finding with relevance for both vaccine design and prevention of inflammatory disorders.

LanguageEnglish (US)
Pages951-966
Number of pages16
JournalJournal of Experimental Medicine
Volume213
Issue number6
DOIs
StatePublished - May 1 2016

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Virus Diseases
T-Lymphocytes
Antigens
Vaccinia virus
Skin
Infection
Population
Recognition (Psychology)
Vaccines
Inflammation

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Local antigen in nonlymphoid tissue promotes resident memory CD8+ T cell formation during viral infection. / Khan, Tahsin N.; Mooster, Jana L.; Kilgore, Augustus M.; Osborn, Jossef F.; Nolz, Jeffrey C.

In: Journal of Experimental Medicine, Vol. 213, No. 6, 01.05.2016, p. 951-966.

Research output: Research - peer-reviewArticle

Khan, Tahsin N. ; Mooster, Jana L. ; Kilgore, Augustus M. ; Osborn, Jossef F. ; Nolz, Jeffrey C./ Local antigen in nonlymphoid tissue promotes resident memory CD8+ T cell formation during viral infection. In: Journal of Experimental Medicine. 2016 ; Vol. 213, No. 6. pp. 951-966
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