Live-cell STED microscopy with genetically encoded biosensor

Natalia M. Mishina, Alexander S. Mishin, Yury Belyaev, Ekaterina A. Bogdanova, Sergey Lukyanov, Carsten Schultz, Vsevolod V. Belousov

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Of the various super-resolution techniques, stimulated emission depletion (STED) microscopy achieves the best temporal resolution at high spatial resolution, enabling live-cell imaging beyond the diffraction limit. However, STED and most other super-resolution imaging methods utilize a particular type of information extractable from the raw data, namely the positions of fluorophores. To expand on the use of super-resolution techniques, we report here the live-cell STED microscopy of a dynamic biosensor. Using the fluorescent H2O2 sensor HyPer2 for subdiffraction imaging, we were able not only to image filaments with superior resolution by localizing emission but also to trace H2O2 produced within living cell by monitoring brightness of the probe. STED microscopy of HyPer2 demonstrates potential utility of FP-based biosensors for super-resolution experiments in situ and in vivo.

Original languageEnglish (US)
Pages (from-to)2928-2932
Number of pages5
JournalNano Letters
Volume15
Issue number5
DOIs
StatePublished - May 13 2015
Externally publishedYes

Fingerprint

Stimulated emission
Biosensing Techniques
stimulated emission
bioinstrumentation
Biosensors
Microscopy
Microscopic examination
depletion
microscopy
cells
Imaging techniques
Fluorophores
Luminance
temporal resolution
Diffraction
Cells
filaments
brightness
spatial resolution
Monitoring

Keywords

  • biosensor
  • hydrogen peroxide
  • HyPer
  • microscopy
  • STED
  • super-resolution imaging

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Mechanical Engineering
  • Medicine(all)

Cite this

Mishina, N. M., Mishin, A. S., Belyaev, Y., Bogdanova, E. A., Lukyanov, S., Schultz, C., & Belousov, V. V. (2015). Live-cell STED microscopy with genetically encoded biosensor. Nano Letters, 15(5), 2928-2932. https://doi.org/10.1021/nl504710z

Live-cell STED microscopy with genetically encoded biosensor. / Mishina, Natalia M.; Mishin, Alexander S.; Belyaev, Yury; Bogdanova, Ekaterina A.; Lukyanov, Sergey; Schultz, Carsten; Belousov, Vsevolod V.

In: Nano Letters, Vol. 15, No. 5, 13.05.2015, p. 2928-2932.

Research output: Contribution to journalArticle

Mishina, NM, Mishin, AS, Belyaev, Y, Bogdanova, EA, Lukyanov, S, Schultz, C & Belousov, VV 2015, 'Live-cell STED microscopy with genetically encoded biosensor', Nano Letters, vol. 15, no. 5, pp. 2928-2932. https://doi.org/10.1021/nl504710z
Mishina NM, Mishin AS, Belyaev Y, Bogdanova EA, Lukyanov S, Schultz C et al. Live-cell STED microscopy with genetically encoded biosensor. Nano Letters. 2015 May 13;15(5):2928-2932. https://doi.org/10.1021/nl504710z
Mishina, Natalia M. ; Mishin, Alexander S. ; Belyaev, Yury ; Bogdanova, Ekaterina A. ; Lukyanov, Sergey ; Schultz, Carsten ; Belousov, Vsevolod V. / Live-cell STED microscopy with genetically encoded biosensor. In: Nano Letters. 2015 ; Vol. 15, No. 5. pp. 2928-2932.
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