Live-cell STED microscopy with genetically encoded biosensor

Natalia M. Mishina, Alexander S. Mishin, Yury Belyaev, Ekaterina A. Bogdanova, Sergey Lukyanov, Carsten Schultz, Vsevolod V. Belousov

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Of the various super-resolution techniques, stimulated emission depletion (STED) microscopy achieves the best temporal resolution at high spatial resolution, enabling live-cell imaging beyond the diffraction limit. However, STED and most other super-resolution imaging methods utilize a particular type of information extractable from the raw data, namely the positions of fluorophores. To expand on the use of super-resolution techniques, we report here the live-cell STED microscopy of a dynamic biosensor. Using the fluorescent H2O2 sensor HyPer2 for subdiffraction imaging, we were able not only to image filaments with superior resolution by localizing emission but also to trace H2O2 produced within living cell by monitoring brightness of the probe. STED microscopy of HyPer2 demonstrates potential utility of FP-based biosensors for super-resolution experiments in situ and in vivo.

Original languageEnglish (US)
Pages (from-to)2928-2932
Number of pages5
JournalNano Letters
Volume15
Issue number5
DOIs
StatePublished - May 13 2015

Keywords

  • HyPer
  • STED
  • biosensor
  • hydrogen peroxide
  • microscopy
  • super-resolution imaging

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanical Engineering

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  • Cite this

    Mishina, N. M., Mishin, A. S., Belyaev, Y., Bogdanova, E. A., Lukyanov, S., Schultz, C., & Belousov, V. V. (2015). Live-cell STED microscopy with genetically encoded biosensor. Nano Letters, 15(5), 2928-2932. https://doi.org/10.1021/nl504710z