Live cell imaging of a fluorescent gentamicin conjugate

Jorge O. Escobedo, Yu Hsuan Chu, Qi Wang, Peter Steyger, Robert M. Strongin

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Understanding cellular mechanisms of ototoxic and nephrotoxic drug uptake, intracellular distribution, and molecular trafficking across cellular barrier systems aids the study of potential uptake blockers that preserve sensory and renal function during critical life-saving therapy. Herein we report the design, synthesis characterization and evaluation of a fluorescent conjugate of the aminoglycoside antibiotic gentamicin. Live cell imaging results show the potential utility of this new material. Related gentamicin conjugates studied to date quench in live kindney cells, and have been largely restricted to use in fixed (delipidated) cells.

Original languageEnglish (US)
Pages (from-to)317-320
Number of pages4
JournalNatural Product Communications
Volume7
Issue number3
StatePublished - 2012

Fingerprint

gentamicin
Gentamicins
image analysis
aminoglycoside antibiotics
uptake mechanisms
Aminoglycosides
renal function
cells
preserves
Anti-Bacterial Agents
Kidney
drugs
therapeutics
synthesis
Pharmaceutical Preparations
Therapeutics

Keywords

  • Aminoglycosides
  • Gentamicin
  • Ototoxic drugs

ASJC Scopus subject areas

  • Drug Discovery
  • Pharmacology
  • Complementary and alternative medicine
  • Plant Science

Cite this

Escobedo, J. O., Chu, Y. H., Wang, Q., Steyger, P., & Strongin, R. M. (2012). Live cell imaging of a fluorescent gentamicin conjugate. Natural Product Communications, 7(3), 317-320.

Live cell imaging of a fluorescent gentamicin conjugate. / Escobedo, Jorge O.; Chu, Yu Hsuan; Wang, Qi; Steyger, Peter; Strongin, Robert M.

In: Natural Product Communications, Vol. 7, No. 3, 2012, p. 317-320.

Research output: Contribution to journalArticle

Escobedo, JO, Chu, YH, Wang, Q, Steyger, P & Strongin, RM 2012, 'Live cell imaging of a fluorescent gentamicin conjugate', Natural Product Communications, vol. 7, no. 3, pp. 317-320.
Escobedo JO, Chu YH, Wang Q, Steyger P, Strongin RM. Live cell imaging of a fluorescent gentamicin conjugate. Natural Product Communications. 2012;7(3):317-320.
Escobedo, Jorge O. ; Chu, Yu Hsuan ; Wang, Qi ; Steyger, Peter ; Strongin, Robert M. / Live cell imaging of a fluorescent gentamicin conjugate. In: Natural Product Communications. 2012 ; Vol. 7, No. 3. pp. 317-320.
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