Liquid Chromatography with Tandem Mass Spectrometry-Based Proteomic Discovery in Aging and Alzheimer's Disease

Thomas J. Montine, Randall L. Woltjer, Catherine Pan, Kathleen S. Montine, Jing Zhang

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Summary: Systems biology offers enormous potential to understand the complexity of human brain aging and neurodegenerative diseases. Proteomics has an important role in these investigations because of its unique strengths and because of the potential central pathogenic contribution of pathological protein to several of these diseases. Here we have reviewed the methods and presented some examples of liquid chromatography-electrospray ionization-tandem mass spectrometry-based proteomics, with and without quantification using isotope-coded affinity tags, in the investigation of aging and Alzheimer's disease. As protocols and methods for improved quantitative high-throughput proteomics constantly improve, this approach will likely continue to provide deeper insight into human brain aging and neurodegenerative diseases.

Original languageEnglish (US)
Pages (from-to)336-343
Number of pages8
JournalNeuroRx
Volume3
Issue number3
DOIs
StatePublished - Jul 2006

Keywords

  • Alzheimer's disease
  • Proteomics
  • cerebrospinal fluid (CSF)
  • neurofibrillary tangles

ASJC Scopus subject areas

  • Pharmacology (medical)

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