Liquid chromatography-tandem mass spectrometry determination of plasma 24S-hydroxycholesterol with chromatographic separation of 25-hydroxycholesterol

Andrea De Barber, Dieter Lütjohann, Louise Merkens, Robert D. Steiner

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Concentrations of circulating 24S-hydroxycholesterol (24SOHChol) are of interest as a practical measure of cholesterol efflux from the human brain. The current method of choice for 24SOHChol quantification is with gas chromatography-mass spectrometry (GC-MS). Liquid chromatography-mass spectrometry (LC-MS) methods to detect 24SOHChol have been described, but they lack rigorous high-performance liquid chromatography (HPLC) separation of a closely eluting isomeric oxysterol, 25-hydroxycholesterol. This is important because 25-hydroxycholesterol can be present in significant amounts and tandem mass spectrometry (MS/MS) cannot completely differentiate 24SOHChol. We describe an LC-MS method with rapid chromatographic separation of the oxysterols to permit accurate determination of plasma 24SOHChol. The availability of an LC-MS method offers advantages such as simplified sample work-up and analysis.

Original languageEnglish (US)
Pages (from-to)151-153
Number of pages3
JournalAnalytical Biochemistry
Volume381
Issue number1
DOIs
StatePublished - Oct 1 2008

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Liquid chromatography
Tandem Mass Spectrometry
Liquid Chromatography
Mass spectrometry
Plasmas
Mass Spectrometry
Gas Chromatography-Mass Spectrometry
High performance liquid chromatography
Gas chromatography
Cholesterol
High Pressure Liquid Chromatography
Brain
24-hydroxycholesterol
25-hydroxycholesterol
Availability
Oxysterols

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Liquid chromatography-tandem mass spectrometry determination of plasma 24S-hydroxycholesterol with chromatographic separation of 25-hydroxycholesterol. / De Barber, Andrea; Lütjohann, Dieter; Merkens, Louise; Steiner, Robert D.

In: Analytical Biochemistry, Vol. 381, No. 1, 01.10.2008, p. 151-153.

Research output: Contribution to journalArticle

De Barber, Andrea ; Lütjohann, Dieter ; Merkens, Louise ; Steiner, Robert D. / Liquid chromatography-tandem mass spectrometry determination of plasma 24S-hydroxycholesterol with chromatographic separation of 25-hydroxycholesterol. In: Analytical Biochemistry. 2008 ; Vol. 381, No. 1. pp. 151-153.
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