TY - JOUR
T1 - Lipopolysaccharide pretreatment modulates the disease course in experimental autoimmune encephalomyelitis
AU - Buenafe, Abigail C.
AU - Bourdette, Dennis N.
N1 - Funding Information:
We would like to acknowledge the technical assistance of Christopher Brannigan, Sophia Cai, and Hanna Choi in this work. This work was supported in part by the Nancy Davis Center Without Walls and the Biomedical Laboratory R&D Service, Department of Veterans' Affairs.
PY - 2007/1
Y1 - 2007/1
N2 - Treatment with the bacterial product lipopolysaccharide (LPS) prior to the induction of experimental autoimmune encephalomyelitis (EAE) consistently led to a delayed onset of disease but not to a reduction in disease severity. T cell proliferation was reduced in LPS-treated mice, due at least in part to a loss in antigen presenting cell function. T cell and macrophage infiltration into the CNS was delayed and TNFα production was diminished in LPS pre-treated mice, consistent with the delay in disease onset. Real-time PCR analysis of gene expression in the CNS of LPS or saline pre-treated mice demonstrated an early induction of TNFα, TGFβ, IFNβ, and SOCS3 in the LPS pre-treated mice. Thus, exposure to LPS prior to EAE induction affects antigen presentation and may modulate the expression of inflammatory regulators that impact the autoimmune disease course.
AB - Treatment with the bacterial product lipopolysaccharide (LPS) prior to the induction of experimental autoimmune encephalomyelitis (EAE) consistently led to a delayed onset of disease but not to a reduction in disease severity. T cell proliferation was reduced in LPS-treated mice, due at least in part to a loss in antigen presenting cell function. T cell and macrophage infiltration into the CNS was delayed and TNFα production was diminished in LPS pre-treated mice, consistent with the delay in disease onset. Real-time PCR analysis of gene expression in the CNS of LPS or saline pre-treated mice demonstrated an early induction of TNFα, TGFβ, IFNβ, and SOCS3 in the LPS pre-treated mice. Thus, exposure to LPS prior to EAE induction affects antigen presentation and may modulate the expression of inflammatory regulators that impact the autoimmune disease course.
KW - Anti-inflammatory mediators
KW - Antigen presentation
KW - EAE
KW - LPS
KW - Multiple sclerosis
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U2 - 10.1016/j.jneuroim.2006.09.004
DO - 10.1016/j.jneuroim.2006.09.004
M3 - Article
C2 - 17055066
AN - SCOPUS:33845886922
VL - 182
SP - 32
EP - 40
JO - Advances in Neuroimmunology
JF - Advances in Neuroimmunology
SN - 0165-5728
IS - 1-2
ER -