Lipopolysaccharide induces disseminated endothelial apoptosis requiring ceramide generation

Adriana Haimovitz-Friedman, Carlos Cordon-Cardo, Shariff Bayoumy, Mark Garzotto, Maureen McLoughlin, Ruth Gallily, Carl K. Edwards, Edward H. Schuchman, Zvi Fuks, Richard Kolesnick

Research output: Contribution to journalArticle

368 Citations (Scopus)

Abstract

The endotoxic shock syndrome is characterized by systemic inflammation, multiple organ damage, circulatory collapse and death. Systemic release of tumor necrosis factor (TNF)-α and other cytokines purportedly mediates this process. However, the primary tissue target remains unidentified. The present studies provide evidence that endotoxic shock results from disseminated endothelial apoptosis. Injection of lipopolysaccharide (LPS), and its putative effector TNF-α, into C57BL/6 mice induced apoptosis in endothelium of intestine, lung, fat and thymus after 6 h, preceding nonendothelial tissue damage. LPS or TNF-α injection was followed within 1 h by tissue generation of the pro-apoptotic lipid ceramide. TNF-binding protein, which protects against LPS-induced death, blocked LPS-induced ceramide generation and endothelial apoptosis, suggesting systemic TNF is required for both responses. Acid sphingomyelinase knockout mice displayed a normal increase in serum TNF-α in response to LPS, yet were protected against endothelial apoptosis and animal death, defining a role for ceramide in mediating the endotoxic response. Furthermore, intravenous injection of basic fibroblast growth factor, which acts as an intravascular survival factor for endothelial cells, blocked LPS-induced ceramide elevation, endothelial apoptosis and animal death, but did not affect LPS-induced elevation of serum TNF-α. These investigations demonstrate that LPS induces a disseminated form of endothelial apoptosis, mediated sequentially by TNF and ceramide generation, and suggest that this cascade is mandatory for evolution of the endotoxic syndrome.

Original languageEnglish (US)
Pages (from-to)1831-1841
Number of pages11
JournalJournal of Experimental Medicine
Volume186
Issue number11
DOIs
StatePublished - Dec 1 1997
Externally publishedYes

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Ceramides
Lipopolysaccharides
Tumor Necrosis Factor-alpha
Apoptosis
Septic Shock
Sphingomyelin Phosphodiesterase
Injections
Fibroblast Growth Factor 2
Serum
Inbred C57BL Mouse
Knockout Mice
Intravenous Injections
Thymus Gland
Endothelium
Intestines
Shock
Carrier Proteins
Endothelial Cells
Fats
Cytokines

ASJC Scopus subject areas

  • Immunology

Cite this

Haimovitz-Friedman, A., Cordon-Cardo, C., Bayoumy, S., Garzotto, M., McLoughlin, M., Gallily, R., ... Kolesnick, R. (1997). Lipopolysaccharide induces disseminated endothelial apoptosis requiring ceramide generation. Journal of Experimental Medicine, 186(11), 1831-1841. https://doi.org/10.1084/jem.186.11.1831

Lipopolysaccharide induces disseminated endothelial apoptosis requiring ceramide generation. / Haimovitz-Friedman, Adriana; Cordon-Cardo, Carlos; Bayoumy, Shariff; Garzotto, Mark; McLoughlin, Maureen; Gallily, Ruth; Edwards, Carl K.; Schuchman, Edward H.; Fuks, Zvi; Kolesnick, Richard.

In: Journal of Experimental Medicine, Vol. 186, No. 11, 01.12.1997, p. 1831-1841.

Research output: Contribution to journalArticle

Haimovitz-Friedman, A, Cordon-Cardo, C, Bayoumy, S, Garzotto, M, McLoughlin, M, Gallily, R, Edwards, CK, Schuchman, EH, Fuks, Z & Kolesnick, R 1997, 'Lipopolysaccharide induces disseminated endothelial apoptosis requiring ceramide generation', Journal of Experimental Medicine, vol. 186, no. 11, pp. 1831-1841. https://doi.org/10.1084/jem.186.11.1831
Haimovitz-Friedman, Adriana ; Cordon-Cardo, Carlos ; Bayoumy, Shariff ; Garzotto, Mark ; McLoughlin, Maureen ; Gallily, Ruth ; Edwards, Carl K. ; Schuchman, Edward H. ; Fuks, Zvi ; Kolesnick, Richard. / Lipopolysaccharide induces disseminated endothelial apoptosis requiring ceramide generation. In: Journal of Experimental Medicine. 1997 ; Vol. 186, No. 11. pp. 1831-1841.
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