Lipopolysaccharide-binding protein in microbial invasion of the amniotic cavity and human parturition

J. Espinoza, R. Romero, T. Chaiworapongsa, J. C. Kim, J. Yoshimatsu, S. Edwin, C. Rathnasabapathy, Jorge Tolosa, A. Donnenfeld, F. Craparo, R. Gomez, E. Bujold

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Objective: Lipopolysaccharide-binding protein (LBP) is an acute-phase protein of predominantly hepatic origin, capable of binding the lipid A fraction of bacterial lipopolysaccharide (LPS). The complex LBP-LPS binds to CD14, and has been implicated in the host response to Gram-negative infection. The purpose of this study was to determine whether microbial invasion of the amniotic cavity (MIAC) and parturition (term and preterm) are associated with changes in the amniotic fluid concentration of LBP. Study design: Amniotic fluid was retrieved by amniocentesis from 343 patients in the following groups: (1) those in mid-trimester with a subsequent normal pregnancy outcome (n = 84); (2) those in mid-trimester with a fetal loss after the procedure (n = 10); (3) those with preterm labor and intact membranes without MIAC who delivered at term (n = 36) or prematurely (n = 52), and those with preterm labor with MIAC (n = 26); (4) those with preterm premature rupture of membranes (PROM) with (n = 26) and without (n = 26) MIAC; and (5) those delivering at term with intact membranes in the absence of MIAC, in labor (n = 52) and not in labor (n = 31). The concentration of LBP in amniotic fluid was determined with a specific and sensitive immunoassay. Non-parametric statistics were used. A p value of <0.05 was considered significant. Results: LBP was detected in 98% (335/343) of the amniotic fluid samples. MIAC was associated with a significant increase in amniotic fluid concentration of LBP in women with preterm labor and intact membranes, but not in preterm PROM. Spontaneous preterm parturition was associated with a significant increase in amniotic fluid concentration of LBP. Patients who had a spontaneous fetal loss after a mid-trimester amniocentesis had a significantly higher median amniotic fluid LBP concentration than those who had a mid-trimester amniocentesis and a normal perinatal outcome. Conclusion: Preterm labor with MIAC and preterm parturition are associated with higher amniotic fluid concentrations of LBP than those with sterile amniotic fluid.

Original languageEnglish (US)
Pages (from-to)313-321
Number of pages9
JournalJournal of Maternal-Fetal and Neonatal Medicine
Volume12
Issue number5
StatePublished - Nov 1 2002
Externally publishedYes

Fingerprint

Amniotic Fluid
Parturition
Premature Obstetric Labor
Amniocentesis
Membranes
Lipopolysaccharides
lipopolysaccharide-binding protein
Lipid A
Acute-Phase Proteins
Pregnancy Outcome
Nonparametric Statistics
Immunoassay
Liver
Infection

Keywords

  • Amniotic fluid
  • Chorioamnionitis
  • Endotoxin
  • Lipopolysaccharide-binding protein
  • Preterm labor
  • Toll-like receptor

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Obstetrics and Gynecology

Cite this

Espinoza, J., Romero, R., Chaiworapongsa, T., Kim, J. C., Yoshimatsu, J., Edwin, S., ... Bujold, E. (2002). Lipopolysaccharide-binding protein in microbial invasion of the amniotic cavity and human parturition. Journal of Maternal-Fetal and Neonatal Medicine, 12(5), 313-321.

Lipopolysaccharide-binding protein in microbial invasion of the amniotic cavity and human parturition. / Espinoza, J.; Romero, R.; Chaiworapongsa, T.; Kim, J. C.; Yoshimatsu, J.; Edwin, S.; Rathnasabapathy, C.; Tolosa, Jorge; Donnenfeld, A.; Craparo, F.; Gomez, R.; Bujold, E.

In: Journal of Maternal-Fetal and Neonatal Medicine, Vol. 12, No. 5, 01.11.2002, p. 313-321.

Research output: Contribution to journalArticle

Espinoza, J, Romero, R, Chaiworapongsa, T, Kim, JC, Yoshimatsu, J, Edwin, S, Rathnasabapathy, C, Tolosa, J, Donnenfeld, A, Craparo, F, Gomez, R & Bujold, E 2002, 'Lipopolysaccharide-binding protein in microbial invasion of the amniotic cavity and human parturition', Journal of Maternal-Fetal and Neonatal Medicine, vol. 12, no. 5, pp. 313-321.
Espinoza J, Romero R, Chaiworapongsa T, Kim JC, Yoshimatsu J, Edwin S et al. Lipopolysaccharide-binding protein in microbial invasion of the amniotic cavity and human parturition. Journal of Maternal-Fetal and Neonatal Medicine. 2002 Nov 1;12(5):313-321.
Espinoza, J. ; Romero, R. ; Chaiworapongsa, T. ; Kim, J. C. ; Yoshimatsu, J. ; Edwin, S. ; Rathnasabapathy, C. ; Tolosa, Jorge ; Donnenfeld, A. ; Craparo, F. ; Gomez, R. ; Bujold, E. / Lipopolysaccharide-binding protein in microbial invasion of the amniotic cavity and human parturition. In: Journal of Maternal-Fetal and Neonatal Medicine. 2002 ; Vol. 12, No. 5. pp. 313-321.
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AU - Espinoza, J.

AU - Romero, R.

AU - Chaiworapongsa, T.

AU - Kim, J. C.

AU - Yoshimatsu, J.

AU - Edwin, S.

AU - Rathnasabapathy, C.

AU - Tolosa, Jorge

AU - Donnenfeld, A.

AU - Craparo, F.

AU - Gomez, R.

AU - Bujold, E.

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N2 - Objective: Lipopolysaccharide-binding protein (LBP) is an acute-phase protein of predominantly hepatic origin, capable of binding the lipid A fraction of bacterial lipopolysaccharide (LPS). The complex LBP-LPS binds to CD14, and has been implicated in the host response to Gram-negative infection. The purpose of this study was to determine whether microbial invasion of the amniotic cavity (MIAC) and parturition (term and preterm) are associated with changes in the amniotic fluid concentration of LBP. Study design: Amniotic fluid was retrieved by amniocentesis from 343 patients in the following groups: (1) those in mid-trimester with a subsequent normal pregnancy outcome (n = 84); (2) those in mid-trimester with a fetal loss after the procedure (n = 10); (3) those with preterm labor and intact membranes without MIAC who delivered at term (n = 36) or prematurely (n = 52), and those with preterm labor with MIAC (n = 26); (4) those with preterm premature rupture of membranes (PROM) with (n = 26) and without (n = 26) MIAC; and (5) those delivering at term with intact membranes in the absence of MIAC, in labor (n = 52) and not in labor (n = 31). The concentration of LBP in amniotic fluid was determined with a specific and sensitive immunoassay. Non-parametric statistics were used. A p value of <0.05 was considered significant. Results: LBP was detected in 98% (335/343) of the amniotic fluid samples. MIAC was associated with a significant increase in amniotic fluid concentration of LBP in women with preterm labor and intact membranes, but not in preterm PROM. Spontaneous preterm parturition was associated with a significant increase in amniotic fluid concentration of LBP. Patients who had a spontaneous fetal loss after a mid-trimester amniocentesis had a significantly higher median amniotic fluid LBP concentration than those who had a mid-trimester amniocentesis and a normal perinatal outcome. Conclusion: Preterm labor with MIAC and preterm parturition are associated with higher amniotic fluid concentrations of LBP than those with sterile amniotic fluid.

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KW - Amniotic fluid

KW - Chorioamnionitis

KW - Endotoxin

KW - Lipopolysaccharide-binding protein

KW - Preterm labor

KW - Toll-like receptor

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