Lineage infidelity of MDA-MB-435 cells: Expression of melanocyte proteins in a breast cancer cell line

Shankar Sellappan, Rebecca Grijalva, Xiaoyan Zhou, Wentao Yang, Menashe Bar Eli, Gordon Mills, Dihua Yu

Research output: Contribution to journalArticle

128 Citations (Scopus)

Abstract

The origin of cell lines is critical in defining cell type-specific biological functions. Several reports (D. T. Ross et al., Nat Genet 2000;24:227-35; G. Ellison et al., J Clin Pathol Mol Pathol 2002;55:294-9) suggested that the MDA-MB-435 cell line, a cell line extensively used for studying breast cancer biology, has a gene expression pattern most compatible with melanocyte origin. However, we demonstrate that MDA-MB-435 cells express breast-specific or epithelial-specific markers. Also, MDA-MB-435 cells were induced to express breast differentiation-specific proteins and secrete milk lipids as observed in other well-established breast cancer cell lines. Notably, MDA-MB-435 cells also expressed melanocyte-specific proteins as did another highly aggressive breast cancer cell line. MDA-MB-435 xenograft tissue sections stained entirely positive for epithelium-specific markers but only partially positive for melanocyte-specific markers. Thus, MDA-MB-435 is most likely a breast epithelial cell line that has undergone lineage infidelity.

Original languageEnglish (US)
Pages (from-to)3479-3485
Number of pages7
JournalCancer Research
Volume64
Issue number10
DOIs
StatePublished - May 15 2004
Externally publishedYes

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Melanocytes
Breast Neoplasms
Cell Line
Proteins
Breast
Viverridae
Milk Proteins
Heterografts
Epithelium
Epithelial Cells
Lipids
Gene Expression

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Lineage infidelity of MDA-MB-435 cells : Expression of melanocyte proteins in a breast cancer cell line. / Sellappan, Shankar; Grijalva, Rebecca; Zhou, Xiaoyan; Yang, Wentao; Eli, Menashe Bar; Mills, Gordon; Yu, Dihua.

In: Cancer Research, Vol. 64, No. 10, 15.05.2004, p. 3479-3485.

Research output: Contribution to journalArticle

Sellappan, Shankar ; Grijalva, Rebecca ; Zhou, Xiaoyan ; Yang, Wentao ; Eli, Menashe Bar ; Mills, Gordon ; Yu, Dihua. / Lineage infidelity of MDA-MB-435 cells : Expression of melanocyte proteins in a breast cancer cell line. In: Cancer Research. 2004 ; Vol. 64, No. 10. pp. 3479-3485.
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