Limiting dilution analysis of interleukin 2 and colony-stimulating factor producer cells in normal and autoimmune mice

Steven Hefeneider, P. J. Conlon, S. K. Dower, C. S. Henney, S. Gillis

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

MRL/MP 1pr-1pr (MRL-1pr) mice spontaneously develop an age-related disease characteristic of human systemic lupus erythematosus (SLE). Old MRL-1pr mice (4 mo of age) develop antibodies to nucleic acids, display immune complex glomerulonephritis, and have a massive T cell-associated lymphadenopathy. In concert with disease development is data showing an age-related loss in interleukin 2 (IL 2) production by mitogen-stimulated lymphoid cells from these mice. The loss of IL 2 production has been suggested to be involved in the onset and/or development of autoimmune disease seen in these animals. In this report, we examined the frequency of both IL 2 and colony-stimulating factor (CSF) producer T cells in the MRL-1pr mouse by using a limiting dilution analysis assay. Our results show that the number of IL 2 and CSF producer cells present in autoimmune animals is similar to the number found in normal control mice. In addition, IL 2 and CSF producer T cells from autoimmune MRL-1pr mice make similar levels of lymphokine activity, as do producer T cells from normal mice. Our data argue against the previously hypothesized role that a paucity of IL 2 production may be involved in the etiology of autoimmune disease.

Original languageEnglish (US)
Pages (from-to)1863-1868
Number of pages6
JournalJournal of Immunology
Volume132
Issue number4
StatePublished - 1984
Externally publishedYes

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Colony-Stimulating Factors
Interleukin-2
T-Lymphocytes
Autoimmune Diseases
Lymphokines
Glomerulonephritis
Antigen-Antibody Complex
Mitogens
Systemic Lupus Erythematosus
Nucleic Acids
Lymphocytes
Antibodies

ASJC Scopus subject areas

  • Immunology

Cite this

Limiting dilution analysis of interleukin 2 and colony-stimulating factor producer cells in normal and autoimmune mice. / Hefeneider, Steven; Conlon, P. J.; Dower, S. K.; Henney, C. S.; Gillis, S.

In: Journal of Immunology, Vol. 132, No. 4, 1984, p. 1863-1868.

Research output: Contribution to journalArticle

Hefeneider, Steven ; Conlon, P. J. ; Dower, S. K. ; Henney, C. S. ; Gillis, S. / Limiting dilution analysis of interleukin 2 and colony-stimulating factor producer cells in normal and autoimmune mice. In: Journal of Immunology. 1984 ; Vol. 132, No. 4. pp. 1863-1868.
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AU - Henney, C. S.

AU - Gillis, S.

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N2 - MRL/MP 1pr-1pr (MRL-1pr) mice spontaneously develop an age-related disease characteristic of human systemic lupus erythematosus (SLE). Old MRL-1pr mice (4 mo of age) develop antibodies to nucleic acids, display immune complex glomerulonephritis, and have a massive T cell-associated lymphadenopathy. In concert with disease development is data showing an age-related loss in interleukin 2 (IL 2) production by mitogen-stimulated lymphoid cells from these mice. The loss of IL 2 production has been suggested to be involved in the onset and/or development of autoimmune disease seen in these animals. In this report, we examined the frequency of both IL 2 and colony-stimulating factor (CSF) producer T cells in the MRL-1pr mouse by using a limiting dilution analysis assay. Our results show that the number of IL 2 and CSF producer cells present in autoimmune animals is similar to the number found in normal control mice. In addition, IL 2 and CSF producer T cells from autoimmune MRL-1pr mice make similar levels of lymphokine activity, as do producer T cells from normal mice. Our data argue against the previously hypothesized role that a paucity of IL 2 production may be involved in the etiology of autoimmune disease.

AB - MRL/MP 1pr-1pr (MRL-1pr) mice spontaneously develop an age-related disease characteristic of human systemic lupus erythematosus (SLE). Old MRL-1pr mice (4 mo of age) develop antibodies to nucleic acids, display immune complex glomerulonephritis, and have a massive T cell-associated lymphadenopathy. In concert with disease development is data showing an age-related loss in interleukin 2 (IL 2) production by mitogen-stimulated lymphoid cells from these mice. The loss of IL 2 production has been suggested to be involved in the onset and/or development of autoimmune disease seen in these animals. In this report, we examined the frequency of both IL 2 and colony-stimulating factor (CSF) producer T cells in the MRL-1pr mouse by using a limiting dilution analysis assay. Our results show that the number of IL 2 and CSF producer cells present in autoimmune animals is similar to the number found in normal control mice. In addition, IL 2 and CSF producer T cells from autoimmune MRL-1pr mice make similar levels of lymphokine activity, as do producer T cells from normal mice. Our data argue against the previously hypothesized role that a paucity of IL 2 production may be involved in the etiology of autoimmune disease.

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