Light-sheet fluorescence imaging to localize cardiac lineage and protein distribution

Yichen Ding, Juhyun Lee, Jianguo Ma, Kevin Sung, Tomohiro Yokota, Neha Singh, Mojdeh Dooraghi, Parinaz Abiri, Yibin Wang, Rajan P. Kulkarni, Atsushi Nakano, Thao P. Nguyen, Peng Fei, Tzung K. Hsiai

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

Light-sheet fluorescence microscopy (LSFM) serves to advance developmental research and regenerative medicine. Coupled with the paralleled advances in fluorescence-friendly tissue clearing technique, our cardiac LSFM enables dual-sided illumination to rapidly uncover the architecture of murine hearts over 10 by 10 by 10 mm 3 in volume; thereby allowing for localizing progenitor differentiation to the cardiomyocyte lineage and AAV9-mediated expression of exogenous transmembrane potassium channels with high contrast and resolution. Without the steps of stitching image columns, pivoting the light-sheet and sectioning the heart mechanically, we establish a holistic strategy for 3-dimentional reconstruction of the "digital murine heart" to assess aberrant cardiac structures as well as the spatial distribution of the cardiac lineages in neonates and ion-channels in adults.

Original languageEnglish (US)
Article number42209
JournalScientific Reports
Volume7
DOIs
StatePublished - Feb 6 2017
Externally publishedYes

ASJC Scopus subject areas

  • General

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