TY - JOUR
T1 - Lifespans of diabetic and non-diabetic fibroblasts in vitro
T2 - Results of replicate determinations
AU - Vracko, Rudolf
AU - McFarland, Bentson H.
PY - 1980
Y1 - 1980
N2 - Cumulative replicative lifespan (CRLS) was determined in replicates for skin fibroblasts from ten non-diabetic and 11 diabetic donors. The results show that CRLS 1. decreases as function of donor age for controls but not for diabetics 2. is not different for random subsites of the same biopsy 3. is not affected by freeze-thawing of either tissues or cells or by the calendar time during which the cells were tested 4. is affected by different lots of fetal bovine sera. The data also shows that variation of CRLS among diabetic donors is greater than among controls and that cells from some diabetics may have decreased CRLS.
AB - Cumulative replicative lifespan (CRLS) was determined in replicates for skin fibroblasts from ten non-diabetic and 11 diabetic donors. The results show that CRLS 1. decreases as function of donor age for controls but not for diabetics 2. is not different for random subsites of the same biopsy 3. is not affected by freeze-thawing of either tissues or cells or by the calendar time during which the cells were tested 4. is affected by different lots of fetal bovine sera. The data also shows that variation of CRLS among diabetic donors is greater than among controls and that cells from some diabetics may have decreased CRLS.
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U2 - 10.1016/0014-4827(80)90502-9
DO - 10.1016/0014-4827(80)90502-9
M3 - Article
C2 - 7428825
AN - SCOPUS:0019127719
SN - 0014-4827
VL - 129
SP - 345
EP - 350
JO - Experimental Cell Research
JF - Experimental Cell Research
IS - 2
ER -