Cumulative replicative lifespan (CRLS) was determined in replicates for skin fibroblasts from ten non-diabetic and 11 diabetic donors. The results show that CRLS 1. decreases as function of donor age for controls but not for diabetics 2. is not different for random subsites of the same biopsy 3. is not affected by freeze-thawing of either tissues or cells or by the calendar time during which the cells were tested 4. is affected by different lots of fetal bovine sera. The data also shows that variation of CRLS among diabetic donors is greater than among controls and that cells from some diabetics may have decreased CRLS.
ASJC Scopus subject areas
- Cell Biology