Leukemia inhibitory factor signaling modulates both central nervous system demyelination and myelin repair

Mark P. Marriott, Ben Emery, Holly S. Cate, Michele D. Binder, Dennis Kemper, Qizhu Wu, Scott Kolbe, Ian R. Gordon, Hong Wang, Gary Egan, Simon Murray, Helmut Butzkueven, Trevor J. Kilpatrick

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

Leukemia inhibitory factor (LIF) receptor signaling limits the severity of inflammatory demyelination in experimental autoimmune encephalomyelitis, a T-cell dependent animal model of multiple sclerosis (MS) [Butzkueven et al. (2002) Nat Med 8:613-619]. To identify whether LIF exerts direct effects within the central nervous system to limit demyelination, we have studied the influence of LIF upon the phenotype of mice challenged with cuprizone, a copper chelator, which produces a toxic oligodendrocytopathy. We find that exogenously administered LIF limits cuprizone-induced demyelination. Knockout mice deficient in LIF exhibit both potentiated demyelination and oligodendrocyte loss after cuprizone challenge, an effect that is ameliorated by exogenous LIF, arguing for a direct beneficial effect of endogenous LIF receptor signaling. Numbers of oligodendrocyte progenitor cells in cuprizone-challenged mice are not influenced by either exogenous LIF or LIF deficiency, arguing for effects directed to the differentiated oligodendrocyte. Studies on the influence of LIF upon remyelination after cuprizone challenge fail to reveal any significant effect of exogenous LIF. The LIF-knockout mice do, however, display impaired remyelination, although oligodendrocyte replenishment, previously identified to occur from the progenitor pool, is not significantly compromised. Thus endogenous LIF receptor signaling is not only protective of oligodendrocytes but can also enhance remyelination, and exogenous LIF has therapeutic potential in limiting the consequences of oligodendrocyte damage.

Original languageEnglish (US)
Pages (from-to)686-698
Number of pages13
JournalGLIA
Volume56
Issue number6
DOIs
StatePublished - Apr 15 2008
Externally publishedYes

Fingerprint

Leukemia Inhibitory Factor
Demyelinating Diseases
Myelin Sheath
Central Nervous System
Cuprizone
Oligodendroglia
OSM-LIF Receptors
Knockout Mice
Autoimmune Experimental Encephalomyelitis
Poisons
Chelating Agents
Multiple Sclerosis
Copper
Stem Cells
Animal Models

Keywords

  • Cuprizone
  • LIF
  • Myelin
  • Oligodendrocyte
  • Oligodendrocyte progenitor
  • Remyelination

ASJC Scopus subject areas

  • Immunology

Cite this

Marriott, M. P., Emery, B., Cate, H. S., Binder, M. D., Kemper, D., Wu, Q., ... Kilpatrick, T. J. (2008). Leukemia inhibitory factor signaling modulates both central nervous system demyelination and myelin repair. GLIA, 56(6), 686-698. https://doi.org/10.1002/glia.20646

Leukemia inhibitory factor signaling modulates both central nervous system demyelination and myelin repair. / Marriott, Mark P.; Emery, Ben; Cate, Holly S.; Binder, Michele D.; Kemper, Dennis; Wu, Qizhu; Kolbe, Scott; Gordon, Ian R.; Wang, Hong; Egan, Gary; Murray, Simon; Butzkueven, Helmut; Kilpatrick, Trevor J.

In: GLIA, Vol. 56, No. 6, 15.04.2008, p. 686-698.

Research output: Contribution to journalArticle

Marriott, MP, Emery, B, Cate, HS, Binder, MD, Kemper, D, Wu, Q, Kolbe, S, Gordon, IR, Wang, H, Egan, G, Murray, S, Butzkueven, H & Kilpatrick, TJ 2008, 'Leukemia inhibitory factor signaling modulates both central nervous system demyelination and myelin repair', GLIA, vol. 56, no. 6, pp. 686-698. https://doi.org/10.1002/glia.20646
Marriott, Mark P. ; Emery, Ben ; Cate, Holly S. ; Binder, Michele D. ; Kemper, Dennis ; Wu, Qizhu ; Kolbe, Scott ; Gordon, Ian R. ; Wang, Hong ; Egan, Gary ; Murray, Simon ; Butzkueven, Helmut ; Kilpatrick, Trevor J. / Leukemia inhibitory factor signaling modulates both central nervous system demyelination and myelin repair. In: GLIA. 2008 ; Vol. 56, No. 6. pp. 686-698.
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