Let there be bioluminescence: Development of a biophotonic imaging platform for in situ analyses of oral biofilms in animal models

Justin Merritt, Hidenobu Senpuku, Jens Kreth

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11 Citations (Scopus)

Abstract

In the current study, we describe a novel biophotonic imaging-based reporter system that is particularly useful for the study of virulence in polymicrobial infections and interspecies interactions within animal models. A suite of luciferase enzymes was compared using three early colonizing species of the human oral flora (Streptococcus mutans, Streptococcus gordonii and Streptococcus sanguinis) to determine the utility of the different reporters for multiplexed imaging studies in vivo. Using the multiplex approach, we were able to track individual species within a dual-species oral infection model in mice with both temporal and spatial resolution. We also demonstrate how biophotonic imaging of multiplexed luciferase reporters could be adapted for real-time quantification of bacterial gene expression in situ. By creating an inducible dual-luciferase expressing reporter strain of S.mutans, we were able to exogenously control and measure expression of nlmAB (encoding the bacteriocin mutacin IV) within mice to assess its importance for the persistence ability of S.mutans in the oral cavity. The imaging system described in the current study circumvents many of the inherent limitations of current animal model systems, which should now make it feasible to test hypotheses that were previously impractical to model.

Original languageEnglish (US)
Pages (from-to)174-190
Number of pages17
JournalEnvironmental Microbiology
Volume18
Issue number1
DOIs
StatePublished - Jan 1 2016

Fingerprint

bioluminescence
Biofilms
Luciferases
biofilm
mouth
luciferase
Animal Models
animal models
image analysis
animal
Streptococcus gordonii
Bacterial Genes
Bacteriocins
Streptococcus mutans
Streptococcus sanguinis
Streptococcus
Coinfection
Virulence
Mouth
bacteriocins

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

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