Lentiviral-mediated transgene expression can potentiate intestinal mesenchymal-epithelial signaling

Adria D. Dismuke, Aimee D. Kohn, Randall T. Moon, Melissa H. Wong

    Research output: Contribution to journalArticle

    3 Scopus citations

    Abstract

    Mesenchymal-epithelial signaling is essential for the development of many organs and is often disrupted in disease. In this study, we demonstrate the use of lentiviral-mediated transgene delivery as an effective approach for ectopic transgene expression and an alternative to generation of transgenic animals. One benefit to this approach is that it can be used independently or in conjunction with established transgenic or knockout animals for studying modulation of mesenchymal-epithelial interactions. To display the power of this approach, we explored ectopic expression of a Wnt ligand in the mouse intestinal mesenchyme and demonstrate its functional influence on the adjacent epithelium. Our findings highlight the efficient use of lentiviral-mediated transgene expression for modulating mesenchymal-epithelial interactions in vivo.

    Original languageEnglish (US)
    Pages (from-to)130-144
    Number of pages15
    JournalBiological Procedures Online
    Volume11
    Issue number1
    DOIs
    StatePublished - Jul 15 2009

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    Keywords

    • Intestine
    • Lentivirus
    • Mesenchymal-epithelial signaling
    • Mouse
    • Transgene expression
    • Wnt signaling

    ASJC Scopus subject areas

    • Biochemistry, Genetics and Molecular Biology(all)

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