Lentiviral-mediated transgene expression can potentiate intestinal mesenchymal-epithelial signaling

Adria D. Dismuke, Aimee D. Kohn, Randall T. Moon, Melissa H. Wong

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Mesenchymal-epithelial signaling is essential for the development of many organs and is often disrupted in disease. In this study, we demonstrate the use of lentiviral-mediated transgene delivery as an effective approach for ectopic transgene expression and an alternative to generation of transgenic animals. One benefit to this approach is that it can be used independently or in conjunction with established transgenic or knockout animals for studying modulation of mesenchymal-epithelial interactions. To display the power of this approach, we explored ectopic expression of a Wnt ligand in the mouse intestinal mesenchyme and demonstrate its functional influence on the adjacent epithelium. Our findings highlight the efficient use of lentiviral-mediated transgene expression for modulating mesenchymal-epithelial interactions in vivo.

Original languageEnglish (US)
Pages (from-to)130-144
Number of pages15
JournalBiological Procedures Online
Volume11
Issue number1
DOIs
StatePublished - 2009

Keywords

  • Intestine
  • Lentivirus
  • Mesenchymal-epithelial signaling
  • Mouse
  • Transgene expression
  • Wnt signaling

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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