Lentiviral-mediated transgene expression can potentiate intestinal mesenchymal-epithelial signaling

Adria D. Dismuke, Aimee D. Kohn, Randall T. Moon, Melissa Wong

    Research output: Contribution to journalArticle

    3 Citations (Scopus)

    Abstract

    Mesenchymal-epithelial signaling is essential for the development of many organs and is often disrupted in disease. In this study, we demonstrate the use of lentiviral-mediated transgene delivery as an effective approach for ectopic transgene expression and an alternative to generation of transgenic animals. One benefit to this approach is that it can be used independently or in conjunction with established transgenic or knockout animals for studying modulation of mesenchymal-epithelial interactions. To display the power of this approach, we explored ectopic expression of a Wnt ligand in the mouse intestinal mesenchyme and demonstrate its functional influence on the adjacent epithelium. Our findings highlight the efficient use of lentiviral-mediated transgene expression for modulating mesenchymal-epithelial interactions in vivo.

    Original languageEnglish (US)
    Pages (from-to)130-144
    Number of pages15
    JournalBiological Procedures Online
    Volume11
    Issue number1
    DOIs
    StatePublished - 2009

    Fingerprint

    Transgenes
    Animals
    Genetically Modified Animals
    Modulation
    Mesoderm
    Ligands
    Epithelium
    Ectopic Gene Expression

    Keywords

    • Intestine
    • Lentivirus
    • Mesenchymal-epithelial signaling
    • Mouse
    • Transgene expression
    • Wnt signaling

    ASJC Scopus subject areas

    • Biochemistry, Genetics and Molecular Biology(all)

    Cite this

    Lentiviral-mediated transgene expression can potentiate intestinal mesenchymal-epithelial signaling. / Dismuke, Adria D.; Kohn, Aimee D.; Moon, Randall T.; Wong, Melissa.

    In: Biological Procedures Online, Vol. 11, No. 1, 2009, p. 130-144.

    Research output: Contribution to journalArticle

    Dismuke, Adria D. ; Kohn, Aimee D. ; Moon, Randall T. ; Wong, Melissa. / Lentiviral-mediated transgene expression can potentiate intestinal mesenchymal-epithelial signaling. In: Biological Procedures Online. 2009 ; Vol. 11, No. 1. pp. 130-144.
    @article{3d1698005ff746c9a4ee791360fec469,
    title = "Lentiviral-mediated transgene expression can potentiate intestinal mesenchymal-epithelial signaling",
    abstract = "Mesenchymal-epithelial signaling is essential for the development of many organs and is often disrupted in disease. In this study, we demonstrate the use of lentiviral-mediated transgene delivery as an effective approach for ectopic transgene expression and an alternative to generation of transgenic animals. One benefit to this approach is that it can be used independently or in conjunction with established transgenic or knockout animals for studying modulation of mesenchymal-epithelial interactions. To display the power of this approach, we explored ectopic expression of a Wnt ligand in the mouse intestinal mesenchyme and demonstrate its functional influence on the adjacent epithelium. Our findings highlight the efficient use of lentiviral-mediated transgene expression for modulating mesenchymal-epithelial interactions in vivo.",
    keywords = "Intestine, Lentivirus, Mesenchymal-epithelial signaling, Mouse, Transgene expression, Wnt signaling",
    author = "Dismuke, {Adria D.} and Kohn, {Aimee D.} and Moon, {Randall T.} and Melissa Wong",
    year = "2009",
    doi = "10.1007/s12575-009-9014-z",
    language = "English (US)",
    volume = "11",
    pages = "130--144",
    journal = "Biological Procedures Online",
    issn = "1480-9222",
    publisher = "BioMed Central",
    number = "1",

    }

    TY - JOUR

    T1 - Lentiviral-mediated transgene expression can potentiate intestinal mesenchymal-epithelial signaling

    AU - Dismuke, Adria D.

    AU - Kohn, Aimee D.

    AU - Moon, Randall T.

    AU - Wong, Melissa

    PY - 2009

    Y1 - 2009

    N2 - Mesenchymal-epithelial signaling is essential for the development of many organs and is often disrupted in disease. In this study, we demonstrate the use of lentiviral-mediated transgene delivery as an effective approach for ectopic transgene expression and an alternative to generation of transgenic animals. One benefit to this approach is that it can be used independently or in conjunction with established transgenic or knockout animals for studying modulation of mesenchymal-epithelial interactions. To display the power of this approach, we explored ectopic expression of a Wnt ligand in the mouse intestinal mesenchyme and demonstrate its functional influence on the adjacent epithelium. Our findings highlight the efficient use of lentiviral-mediated transgene expression for modulating mesenchymal-epithelial interactions in vivo.

    AB - Mesenchymal-epithelial signaling is essential for the development of many organs and is often disrupted in disease. In this study, we demonstrate the use of lentiviral-mediated transgene delivery as an effective approach for ectopic transgene expression and an alternative to generation of transgenic animals. One benefit to this approach is that it can be used independently or in conjunction with established transgenic or knockout animals for studying modulation of mesenchymal-epithelial interactions. To display the power of this approach, we explored ectopic expression of a Wnt ligand in the mouse intestinal mesenchyme and demonstrate its functional influence on the adjacent epithelium. Our findings highlight the efficient use of lentiviral-mediated transgene expression for modulating mesenchymal-epithelial interactions in vivo.

    KW - Intestine

    KW - Lentivirus

    KW - Mesenchymal-epithelial signaling

    KW - Mouse

    KW - Transgene expression

    KW - Wnt signaling

    UR - http://www.scopus.com/inward/record.url?scp=77649272217&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=77649272217&partnerID=8YFLogxK

    U2 - 10.1007/s12575-009-9014-z

    DO - 10.1007/s12575-009-9014-z

    M3 - Article

    C2 - 19597903

    AN - SCOPUS:77649272217

    VL - 11

    SP - 130

    EP - 144

    JO - Biological Procedures Online

    JF - Biological Procedures Online

    SN - 1480-9222

    IS - 1

    ER -