Lens crystallins in aqueous and vitreous humor in selenite overdose cataract

Hiroshi Watanabe, Thomas R. Shearer

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

The purpose of this research was to determine if crystallins were released from the lens into the aqueous and vitreous humor in selenite-overdose cataract. At 20 days post injection, aqueous and vitreous were obtained from lenses containing nuclear cataract or "mature" cataract (nuclear and cortical opacities). Humors were analyzed by direct ELISA and immunoblotting using antibodies against lens crystallins. Both types of selenite cataract showed increased α β and γcrystallins in aqueous and vitreous. Increases in crystallins in vitreous humor were larger than in aqueous humor, possibly because of slower turnover of vitreous. Extensive membrane damage in selenite cataract probably caused leakage of crystallins from the lens to aqueous and vitreous humors. This model may be useful for studies on the immunologic response of the body to lens crystallins released by cataract.

Original languageEnglish (US)
Pages (from-to)479-486
Number of pages8
JournalCurrent Eye Research
Volume8
Issue number5
DOIs
StatePublished - Jan 1 1989

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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