Lens crystallins in aqueous and vitreous humor in selenite overdose cataract

Hiroshi Watanabe, Thomas (Tom) Shearer

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The purpose of this research was to determine if crystallins were released from the lens into the aqueous and vitreous humor in selenite-overdose cataract. At 20 days post injection, aqueous and vitreous were obtained from lenses containing nuclear cataract or "mature" cataract (nuclear and cortical opacities). Humors were analyzed by direct ELISA and immunoblotting using antibodies against lens crystallins. Both types of selenite cataract showed increased α β and γcrystallins in aqueous and vitreous. Increases in crystallins in vitreous humor were larger than in aqueous humor, possibly because of slower turnover of vitreous. Extensive membrane damage in selenite cataract probably caused leakage of crystallins from the lens to aqueous and vitreous humors. This model may be useful for studies on the immunologic response of the body to lens crystallins released by cataract.

Original languageEnglish (US)
Pages (from-to)479-486
Number of pages8
JournalCurrent Eye Research
Volume8
Issue number5
DOIs
StatePublished - 1989

Fingerprint

Selenious Acid
Vitreous Body
Crystallins
Aqueous Humor
Cataract
Lenses
Wit and Humor
Immunoblotting
Enzyme-Linked Immunosorbent Assay
Injections
Membranes
Antibodies
Research

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Lens crystallins in aqueous and vitreous humor in selenite overdose cataract. / Watanabe, Hiroshi; Shearer, Thomas (Tom).

In: Current Eye Research, Vol. 8, No. 5, 1989, p. 479-486.

Research output: Contribution to journalArticle

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