Leishmania donovani singly deficient in HGPRT, APRT or XPRT are viable in vitro and within mammalian macrophages

Jan M. Boitz, Buddy Ullman

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Leishmania species express three phosphoribosyltransferase enzymes, hypoxanthine-guanine phosphoribosyltransferase (HGPRT), adenine phosphoribosyltransferase (APRT), and xanthine phosphoribosyltransferase (XPRT), which enable this genus to acquire purine nutrients from their hosts. To test whether any of these enzymes is essential for viability, transformation into amastigotes, and infectivity and proliferation within mammalian macrophages, Δhgprt, Δaprt, and Δxprt null mutants were created by targeted gene replacement within a virulent background of Leishmania donovani. Each of the three knockout strains was viable as promastigotes and axenic amastigotes and capable of maintaining an infection in bone marrow-derived murine macrophages. These data support the hypothesis that none of the three phosphoribosyltransferases is essential for purine salvage or viability by itself and that purine salvage occurs through multiple anabolic routes in both parasite life cycle stages. In addition these studies revealed the presence of an adenine aminohydrolase enzyme in L. donovani axenic amastigotes, an activity previously thought to be restricted to promastigotes.

Original languageEnglish (US)
Pages (from-to)24-30
Number of pages7
JournalMolecular and Biochemical Parasitology
Volume148
Issue number1
DOIs
StatePublished - Jul 2006

Fingerprint

Adenine Phosphoribosyltransferase
Hypoxanthine Phosphoribosyltransferase
Leishmania donovani
Macrophages
adenine deaminase
Enzymes
Leishmania
Life Cycle Stages
Parasites
Food
Infection
Genes
In Vitro Techniques
purine
xanthine phosphoribosyltransferase

Keywords

  • Infectivity
  • Metabolism
  • Phosphoribosyltransferases
  • Purines

ASJC Scopus subject areas

  • Molecular Biology
  • Parasitology

Cite this

Leishmania donovani singly deficient in HGPRT, APRT or XPRT are viable in vitro and within mammalian macrophages. / Boitz, Jan M.; Ullman, Buddy.

In: Molecular and Biochemical Parasitology, Vol. 148, No. 1, 07.2006, p. 24-30.

Research output: Contribution to journalArticle

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