Abstract
The hypomethylating agent 5-fluoro-2′-deoxycytidine (FdCyd, NSC 48006) is being evaluated clinically both via the intravenous route and via the oral route in combination with 3,4,5,6-tetrahydrouridine (THU), a potent inhibitor of FdCyd catabolism. To determine the pharmacokinetics of FdCyd and downstream metabolites, we developed and validated an LC–MS/MS assay for the quantitation of FdCyd, 5-fluoro-2′-deoxyuridine (FdUrd), and 5-fluorouracil (FU) in 0.2 mL human plasma. After acetonitrile protein precipitation, the sample was split and separate chromatography was achieved for FdCyd with a Synergi Polar-RP column and for FdUrd and FU with a Shodex Asahipak NH2P-50 2D column. Gradients of 0.1% acetic acid in acetonitrile and water were used. Detection with a Quattromicro quadrupole mass spectrometer with electrospray ionization in positive-ion (FdCyd) or negative-ion (FdUrd and FU) multiple reaction monitoring (MRM) mode. The assay was linear from 5 to 3000 ng/mL for all three analytes and proved to be accurate (96.7-105.5%) and precise (<8.1%CV), and fulfilled FDA criteria for bioanalytical method validation. We demonstrated the suitability of this assay for measuring FdCyd and metabolites FdUrd and FU in plasma from a patient who was administered 120 mg PO FdCyd 30 min after 3000 mg THU. Our LC–MS/MS assay will be an essential tool to further define the pharmacology of FdCyd in ongoing and future studies.
Original language | English (US) |
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Pages (from-to) | 359-366 |
Number of pages | 8 |
Journal | Journal of Pharmaceutical and Biomedical Analysis |
Volume | 129 |
DOIs | |
State | Published - Sep 10 2016 |
Externally published | Yes |
Keywords
- Assay
- FdCyd
- Metabolites
- Tandem mass spectrometry
- Validation
ASJC Scopus subject areas
- Analytical Chemistry
- Pharmaceutical Science
- Drug Discovery
- Spectroscopy
- Clinical Biochemistry