LCAT inhibitors interfere with the enzymatic determination of cholesterol and triglycerides

William S. Harris, Alan Rayford

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

5,5-Dithio bis-(2-nitrobenzoic acid) (DTNB) and p-chloromercuriphenylsulfonic acid (PCMPS) are well-known sulfhydryl inhibitors that are used to inhibit lecithin: cholesterol acyltransferase (LCAT). They were each found to interfere with the enzymatic assays of cholesterol and triglycerides. DTNB falsely reduced the measured plasma cholesterol content, and falsely increased triglyceride readings. The inteference with the triglyceride assay could be largely prevented by blanking for glycerol. PCMPS had only a slight effect on the cholesterol assay, but falsely lowered the triglyceride readings to a great extent, even with glycerol-blanking. Thus, these inhibitors should be avoided when plasma samples are to be enzymatically analyzed for cholesterol or triglycerides.

Original languageEnglish (US)
Pages (from-to)341-343
Number of pages3
JournalLipids
Volume25
Issue number6
DOIs
StatePublished - Jun 1 1990

ASJC Scopus subject areas

  • Biochemistry
  • Organic Chemistry
  • Cell Biology

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