Abstract
Background: Balloon angioplasty damages endothelial cells and stimulates smooth muscle cell proliferation. The effects of local cytotoxic drug therapy on formation of neointima and late endothelial function are not known. This study was designed to determine whether direct infusion of mitomycin C via a microporous balloon catheter could significantly reduce formation of neointima after angioplasty. Furthermore, we investigated whether endothelial cell function is normal 7 weeks after the initial microporous angioplasty procedure. Methods: In 34 New Zealand white rabbits, bilateral external iliac arteries underwent balloon angioplasty, followed by either high-dose (0.66 mg/kg) or low-dose (0.025 mg/kg) mitomycin C in one iliac artery and saline infusion in the contralateral artery, and a control group was given saline in both vessels. Formation of neointima was measured in the iliac arteries after 7 weeks by morphometry. Before sacrifice of 17 'angioplasty' rabbits and three undamaged rabbits, graded doses of acetylcholine and isosorbide dinitrate were infused in the distal aorta, and the iliac artery diameter was measured by computerized quantitative angiography. Results: No significant differences in the absolute area of the intima or the intima: media ratio were demonstrated between control arteries and arteries that were directly infused with either high-dose or low-dose mitomycin. However, within the high-dose group, the mitomycin-treated vessel had a significantly lesser extent of formation of intimal hyperplasia (0.17±0.03 versus 0.27±0.03 mm2, P
| Original language | English (US) |
|---|---|
| Pages (from-to) | 633-641 |
| Number of pages | 9 |
| Journal | Coronary Artery Disease |
| Volume | 5 |
| Issue number | 7 |
| State | Published - 1994 |
| Externally published | Yes |
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Keywords
- angioplasty
- antiproliferatives
- endothelial function
- neointimal hyperplasia
- restenosis
ASJC Scopus subject areas
- Cardiology and Cardiovascular Medicine
Cite this
Late effects of locally delivered mitomycin C on formation of neointima and on vasomotor response to acetylcholine. / Strauss, B. H.; Wilson, R. A.; Van Houten, R.; Van Suylen, R. J.; Murphy, Edward; Escaned, J.; Verdouw, P. D.; Serruys, P. W.; Van der Giessen, W. J.
In: Coronary Artery Disease, Vol. 5, No. 7, 1994, p. 633-641.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Late effects of locally delivered mitomycin C on formation of neointima and on vasomotor response to acetylcholine
AU - Strauss, B. H.
AU - Wilson, R. A.
AU - Van Houten, R.
AU - Van Suylen, R. J.
AU - Murphy, Edward
AU - Escaned, J.
AU - Verdouw, P. D.
AU - Serruys, P. W.
AU - Van der Giessen, W. J.
PY - 1994
Y1 - 1994
N2 - Background: Balloon angioplasty damages endothelial cells and stimulates smooth muscle cell proliferation. The effects of local cytotoxic drug therapy on formation of neointima and late endothelial function are not known. This study was designed to determine whether direct infusion of mitomycin C via a microporous balloon catheter could significantly reduce formation of neointima after angioplasty. Furthermore, we investigated whether endothelial cell function is normal 7 weeks after the initial microporous angioplasty procedure. Methods: In 34 New Zealand white rabbits, bilateral external iliac arteries underwent balloon angioplasty, followed by either high-dose (0.66 mg/kg) or low-dose (0.025 mg/kg) mitomycin C in one iliac artery and saline infusion in the contralateral artery, and a control group was given saline in both vessels. Formation of neointima was measured in the iliac arteries after 7 weeks by morphometry. Before sacrifice of 17 'angioplasty' rabbits and three undamaged rabbits, graded doses of acetylcholine and isosorbide dinitrate were infused in the distal aorta, and the iliac artery diameter was measured by computerized quantitative angiography. Results: No significant differences in the absolute area of the intima or the intima: media ratio were demonstrated between control arteries and arteries that were directly infused with either high-dose or low-dose mitomycin. However, within the high-dose group, the mitomycin-treated vessel had a significantly lesser extent of formation of intimal hyperplasia (0.17±0.03 versus 0.27±0.03 mm2, P
AB - Background: Balloon angioplasty damages endothelial cells and stimulates smooth muscle cell proliferation. The effects of local cytotoxic drug therapy on formation of neointima and late endothelial function are not known. This study was designed to determine whether direct infusion of mitomycin C via a microporous balloon catheter could significantly reduce formation of neointima after angioplasty. Furthermore, we investigated whether endothelial cell function is normal 7 weeks after the initial microporous angioplasty procedure. Methods: In 34 New Zealand white rabbits, bilateral external iliac arteries underwent balloon angioplasty, followed by either high-dose (0.66 mg/kg) or low-dose (0.025 mg/kg) mitomycin C in one iliac artery and saline infusion in the contralateral artery, and a control group was given saline in both vessels. Formation of neointima was measured in the iliac arteries after 7 weeks by morphometry. Before sacrifice of 17 'angioplasty' rabbits and three undamaged rabbits, graded doses of acetylcholine and isosorbide dinitrate were infused in the distal aorta, and the iliac artery diameter was measured by computerized quantitative angiography. Results: No significant differences in the absolute area of the intima or the intima: media ratio were demonstrated between control arteries and arteries that were directly infused with either high-dose or low-dose mitomycin. However, within the high-dose group, the mitomycin-treated vessel had a significantly lesser extent of formation of intimal hyperplasia (0.17±0.03 versus 0.27±0.03 mm2, P
KW - angioplasty
KW - antiproliferatives
KW - endothelial function
KW - neointimal hyperplasia
KW - restenosis
UR - http://www.scopus.com/inward/record.url?scp=0027981037&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027981037&partnerID=8YFLogxK
M3 - Article
VL - 5
SP - 633
EP - 641
JO - Coronary Artery Disease
JF - Coronary Artery Disease
SN - 0954-6928
IS - 7
ER -