Laser tweezer controlled solid immersion lens for high resolution imaging in microfluidic and biological samples

Aaron L. Birkbeck, Sanja Zlatanovic, Mihrimah Ozkan, Sadik Esener

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A novel technique is presented which integrates the capacity of a laser tweezer to optically trap and manipulate objects in three-dimensions with the resolution-enhanced imaging capabilities of a solid immersion lens (SIL). Up to now, solid immersion lens imaging systems have relied upon cantilever-mounted SILs that are difficult to integrate into microfluidic systems and require an extra alignment step with external optics. As an alternative to the current state-of-art, we introduce a device that consists of a free-floating SIL and a laser optical tweezer. In our design, the optical tweezer, created by focusing a laser beam through high numerical aperture microscope objective, acts in a two-fold manner: both as a trapping beam for the positioning and alignment of the SIL and as an near-field scanning beam to image the sample through the SIL. Combining the alignment, positioning, and imaging functions into a single device allows for the direct integration of a high resolution imaging system into microfluidic and biological environments.

Original languageEnglish (US)
Pages (from-to)76-84
Number of pages9
JournalUnknown Journal
Volume5275
DOIs
StatePublished - 2004
Externally publishedYes

Fingerprint

Optical Tweezers
Microfluidics
Immersion
submerging
Lenses
lenses
Imaging techniques
Lasers
high resolution
Optical tweezers
lasers
alignment
Imaging systems
positioning
Equipment and Supplies
numerical aperture
floating
Laser beams
Optics
near fields

Keywords

  • Laser tweezer
  • Microfluidics
  • Near-field solid immersion microscopy
  • Solid immersion lens (SIL)

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

Laser tweezer controlled solid immersion lens for high resolution imaging in microfluidic and biological samples. / Birkbeck, Aaron L.; Zlatanovic, Sanja; Ozkan, Mihrimah; Esener, Sadik.

In: Unknown Journal, Vol. 5275, 2004, p. 76-84.

Research output: Contribution to journalArticle

Birkbeck, Aaron L. ; Zlatanovic, Sanja ; Ozkan, Mihrimah ; Esener, Sadik. / Laser tweezer controlled solid immersion lens for high resolution imaging in microfluidic and biological samples. In: Unknown Journal. 2004 ; Vol. 5275. pp. 76-84.
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