Laser tweezer controlled solid immersion lens for high resolution imaging in microfluidic and biological samples

Aaron L. Birkbeck, Sanja Zlatanovic, Mihrimah Ozkan, Sadik C. Esener

Research output: Contribution to journalConference article

3 Scopus citations

Abstract

A novel technique is presented which integrates the capacity of a laser tweezer to optically trap and manipulate objects in three-dimensions with the resolution-enhanced imaging capabilities of a solid immersion lens (SIL). Up to now, solid immersion lens imaging systems have relied upon cantilever-mounted SILs that are difficult to integrate into microfluidic systems and require an extra alignment step with external optics. As an alternative to the current state-of-art, we introduce a device that consists of a free-floating SIL and a laser optical tweezer. In our design, the optical tweezer, created by focusing a laser beam through high numerical aperture microscope objective, acts in a two-fold manner: both as a trapping beam for the positioning and alignment of the SIL and as an near-field scanning beam to image the sample through the SIL. Combining the alignment, positioning, and imaging functions into a single device allows for the direct integration of a high resolution imaging system into microfluidic and biological environments.

Original languageEnglish (US)
Pages (from-to)76-84
Number of pages9
JournalProceedings of SPIE - The International Society for Optical Engineering
Volume5275
DOIs
StatePublished - Jun 1 2004
Externally publishedYes
EventBioMEMS and Nanotechnology - Perth, WA, Australia
Duration: Dec 10 2003Dec 12 2003

Keywords

  • Laser tweezer
  • Microfluidics
  • Near-field solid immersion microscopy
  • Solid immersion lens (SIL)

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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