Large complex globular domains of Type VII procollagen contribute to the structure of anchoring fibrils

G. P. Lunstrum, Lynn Sakai, D. R. Keene, N. P. Morris, R. E. Burgeson

Research output: Contribution to journalArticle

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Abstract

Type VII collagen, in the form of an antiparallel dimer, is a major protein component of anchoring fibrils. The ultrastructural appearance of these fibrils suggests that they may serve to anchor the basement membrane zone to the underlying connective tissue matrix. We report here the identification and initial characterization of Type VII procollagen, recovered from the media of epidermoid carcinoma cell cultures. Immunoblotting using monospecific antibodies to Type VII procollagen identifies a single, homogeneous band of at least M(r) 320,000 following disulfide bond reduction. This chain contains 170 kDa of collagen triple helix and 150 kDa of non-helical domain at the carboxyl terminus. Pepsin digestion of this material yields Type VII collagen identical to that isolated from whole tissue and a series of quasi-stable peptides derived from the carboxyl-terminal region. Cell extracts contain procollagen chains identical in size to those secreted into the media. There is no evidence for processing of this material in cell culture. Partial purification by velocity sedimentation and transmission electron microscopic observation following rotary shadowing reveals both monomers (426 nm) and dimers (785 nm). Dimers are antiparallel and interact through 60-nm overlap, with amino-terminal globular domains present at the ends of the overlap. The multi-domain carboxyl-terminal region appears as three similar arms originating from a centralized globular region adjacent to the collagen helix. The carboxyl globular domain is present in whole tissue and may participate in the unique fibril form of this collagen. The amino-terminal globule may function in the antiparallel assembly of dimers.

Original languageEnglish (US)
Pages (from-to)9042-9048
Number of pages7
JournalJournal of Biological Chemistry
Volume261
Issue number19
StatePublished - Dec 1 1986
Externally publishedYes

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Collagen Type VII
Dimers
Collagen
Tissue
Cell culture
Cell Culture Techniques
Procollagen
Pepsin A
Cell Extracts
Anchors
Sedimentation
Immunoblotting
Basement Membrane
Disulfides
Connective Tissue
Purification
Digestion
Squamous Cell Carcinoma
Monomers
Electrons

ASJC Scopus subject areas

  • Biochemistry

Cite this

Large complex globular domains of Type VII procollagen contribute to the structure of anchoring fibrils. / Lunstrum, G. P.; Sakai, Lynn; Keene, D. R.; Morris, N. P.; Burgeson, R. E.

In: Journal of Biological Chemistry, Vol. 261, No. 19, 01.12.1986, p. 9042-9048.

Research output: Contribution to journalArticle

Lunstrum, G. P. ; Sakai, Lynn ; Keene, D. R. ; Morris, N. P. ; Burgeson, R. E. / Large complex globular domains of Type VII procollagen contribute to the structure of anchoring fibrils. In: Journal of Biological Chemistry. 1986 ; Vol. 261, No. 19. pp. 9042-9048.
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