Lanthanide-Binding Tags for 3D X-ray Imaging of Proteins in Cells at Nanoscale Resolution

Tiffany W. Victor, Katherine H. O'Toole, Lindsey M. Easthon, Mingyuan Ge, Randy J. Smith, Xiaojing Huang, Hanfei Yan, Yong S. Chu, Si Chen, Doga Gursoy, Martina Ralle, Barbara Imperiali, Karen N. Allen, Lisa M. Miller

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

We report the application of lanthanide-binding tags (LBTs) for two- and three-dimensional X-ray imaging of individual proteins in cells with a sub-15 nm beam. The method combines encoded LBTs, which are tags of minimal size (ca. 15-20 amino acids) affording high-affinity lanthanide ion binding, and X-ray fluorescence microscopy (XFM). This approach enables visualization of LBT-tagged proteins while simultaneously measuring the elemental distribution in cells at a spatial resolution necessary for visualizing cell membranes and eukaryotic subcellular organelles.

Original languageEnglish (US)
Pages (from-to)2145-2149
Number of pages5
JournalJournal of the American Chemical Society
Volume142
Issue number5
DOIs
StatePublished - Feb 5 2020

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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