Lanthanide-Binding Tags for 3D X-ray Imaging of Proteins in Cells at Nanoscale Resolution

Tiffany W. Victor; Katherine H. O'Toole; Lindsey M. Easthon; Mingyuan Ge; Randy J. Smith; Xiaojing Huang; Hanfei Yan; Yong S. Chu; Si Chen; Doga Gursoy; Martina Ralle; Barbara Imperiali; Karen N. Allen; Lisa M. Miller

doi:10.1021/jacs.9b11571

Lanthanide-Binding Tags for 3D X-ray Imaging of Proteins in Cells at Nanoscale Resolution

Tiffany W. Victor, Katherine H. O'Toole, Lindsey M. Easthon, Mingyuan Ge, Randy J. Smith, Xiaojing Huang, Hanfei Yan, Yong S. Chu, Si Chen, Doga Gursoy, Martina Ralle, Barbara Imperiali, Karen N. Allen, Lisa M. Miller

Molecular and Medical Genetics

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

We report the application of lanthanide-binding tags (LBTs) for two- and three-dimensional X-ray imaging of individual proteins in cells with a sub-15 nm beam. The method combines encoded LBTs, which are tags of minimal size (ca. 15-20 amino acids) affording high-affinity lanthanide ion binding, and X-ray fluorescence microscopy (XFM). This approach enables visualization of LBT-tagged proteins while simultaneously measuring the elemental distribution in cells at a spatial resolution necessary for visualizing cell membranes and eukaryotic subcellular organelles.

Original language	English (US)
Pages (from-to)	2145-2149
Number of pages	5
Journal	Journal of the American Chemical Society
Volume	142
Issue number	5
DOIs	https://doi.org/10.1021/jacs.9b11571
State	Published - Feb 5 2020

ASJC Scopus subject areas

Catalysis
General Chemistry
Biochemistry
Colloid and Surface Chemistry

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Victor, T. W., O'Toole, K. H., Easthon, L. M., Ge, M., Smith, R. J., Huang, X., Yan, H., Chu, Y. S., Chen, S., Gursoy, D., Ralle, M., Imperiali, B., Allen, K. N., & Miller, L. M. (2020). Lanthanide-Binding Tags for 3D X-ray Imaging of Proteins in Cells at Nanoscale Resolution. Journal of the American Chemical Society, 142(5), 2145-2149. https://doi.org/10.1021/jacs.9b11571

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abstract = "We report the application of lanthanide-binding tags (LBTs) for two- and three-dimensional X-ray imaging of individual proteins in cells with a sub-15 nm beam. The method combines encoded LBTs, which are tags of minimal size (ca. 15-20 amino acids) affording high-affinity lanthanide ion binding, and X-ray fluorescence microscopy (XFM). This approach enables visualization of LBT-tagged proteins while simultaneously measuring the elemental distribution in cells at a spatial resolution necessary for visualizing cell membranes and eukaryotic subcellular organelles.",

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AU - Victor, Tiffany W.

AU - O'Toole, Katherine H.

AU - Easthon, Lindsey M.

AU - Ge, Mingyuan

AU - Smith, Randy J.

AU - Huang, Xiaojing

AU - Yan, Hanfei

AU - Chu, Yong S.

AU - Chen, Si

AU - Gursoy, Doga

AU - Ralle, Martina

AU - Imperiali, Barbara

AU - Allen, Karen N.

AU - Miller, Lisa M.

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Lanthanide-Binding Tags for 3D X-ray Imaging of Proteins in Cells at Nanoscale Resolution

Abstract

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