@article{77ce1183b1344eaaaaa3882433ff55e3,
title = "Lanthanide-Binding Tags for 3D X-ray Imaging of Proteins in Cells at Nanoscale Resolution",
abstract = "We report the application of lanthanide-binding tags (LBTs) for two- and three-dimensional X-ray imaging of individual proteins in cells with a sub-15 nm beam. The method combines encoded LBTs, which are tags of minimal size (ca. 15-20 amino acids) affording high-affinity lanthanide ion binding, and X-ray fluorescence microscopy (XFM). This approach enables visualization of LBT-tagged proteins while simultaneously measuring the elemental distribution in cells at a spatial resolution necessary for visualizing cell membranes and eukaryotic subcellular organelles.",
author = "Victor, {Tiffany W.} and O'Toole, {Katherine H.} and Easthon, {Lindsey M.} and Mingyuan Ge and Smith, {Randy J.} and Xiaojing Huang and Hanfei Yan and Chu, {Yong S.} and Si Chen and Doga Gursoy and Martina Ralle and Barbara Imperiali and Allen, {Karen N.} and Miller, {Lisa M.}",
note = "Funding Information: This work was supported by the United States Department of Energy, Office of Biological and Environmental Research, as part of the “Environment Sensing and Response” Scientific Focus Area of the BER Genomic Science Program. T.W.V. was partially supported by the National Institutes of Health T32 Grant 5T32GM092714 and a Director{\textquoteright}s Postdoctoral Fellowship at NSLS-II. B.I. and K.N.A. were supported by NSF MCB-1615252 and MCB-1614608, respectively. This research used beamline 3-ID (HXN) at NSLS-II, a U.S. Department of Energy (DOE) Office of Science User Facility operated for the DOE Office of Science by Brookhaven National Laboratory under Contract No. DE-SC0012704. This research used the BNP beamline at APS, a U.S. DOE Office of Science User Facility operated for the DOE Office of Science by Argonne National Laboratory under Contract No. DE-AC02-06CH11357. Publisher Copyright: Copyright {\textcopyright} 2020 American Chemical Society.",
year = "2020",
month = feb,
day = "5",
doi = "10.1021/jacs.9b11571",
language = "English (US)",
volume = "142",
pages = "2145--2149",
journal = "Journal of the American Chemical Society",
issn = "0002-7863",
publisher = "American Chemical Society",
number = "5",
}