Lactoferrin content of peripheral blood cells

Robert (Rob) Bennett, T. Kokocinski

    Research output: Contribution to journalArticle

    85 Citations (Scopus)

    Abstract

    The peripheral blood of 60 normal adults was separated into plasma, red cells, neutrophils, lymphocytes, monocytes and platelets. Lactoferrin concentrations were measured in the plasma and cell extracts and compared to those of lysozyme. The neutrophil lactoferrin content in males and post-menopausal females was found to be significantly higher than in pre-menopausal females. A small amount of lactoferrin was found in association with monocytes, but not with lymphocytes, erythrocytes and platelets. Neutrophil lysozyme concentrations did not exhibit any variation with sex and age; but the level in monocytes was higher than that in neutrophils. No correlation was observed between individual neutrophil lactoferrin values and the plasma level. Immunofluorescent studies showed neutrophils to have a lobulated pattern suggestive of nuclear staining. Monocytes did not show direct staining, but exhibited a peripheral pattern after prior exposure to lactoferrin - confirming the existence of a surface receptor. Gel chromatography indicated that neutrophil lactoferrin is in a polymerized or complexed form which elutes with the void volume on Sephadex G-200; serum lactoferrin consists of two forms, one of which also elutes with the void volume on Sephadex G-200.

    Original languageEnglish (US)
    Pages (from-to)509-521
    Number of pages13
    JournalBritish Journal of Haematology
    Volume39
    Issue number4
    StatePublished - 1978

    Fingerprint

    Lactoferrin
    Blood Cells
    Neutrophils
    Monocytes
    Muramidase
    Plasma Cells
    Blood Platelets
    Lymphocytes
    Staining and Labeling
    Cell Extracts
    Gel Chromatography
    Erythrocytes
    Serum

    ASJC Scopus subject areas

    • Hematology

    Cite this

    Bennett, R. R., & Kokocinski, T. (1978). Lactoferrin content of peripheral blood cells. British Journal of Haematology, 39(4), 509-521.

    Lactoferrin content of peripheral blood cells. / Bennett, Robert (Rob); Kokocinski, T.

    In: British Journal of Haematology, Vol. 39, No. 4, 1978, p. 509-521.

    Research output: Contribution to journalArticle

    Bennett, RR & Kokocinski, T 1978, 'Lactoferrin content of peripheral blood cells', British Journal of Haematology, vol. 39, no. 4, pp. 509-521.
    Bennett, Robert (Rob) ; Kokocinski, T. / Lactoferrin content of peripheral blood cells. In: British Journal of Haematology. 1978 ; Vol. 39, No. 4. pp. 509-521.
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