Lack of prolactin receptor signaling in mice results in lactotroph proliferation and prolactinomas by dopamine-dependent and -independent mechanisms

Kathryn Schuff, Shane T. Hentges, Michele A. Kelly, Nadine Binart, Paul A. Kelly, P. Michael Iuvone, Sylvia L. Asa, Malcolm J. Low

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Abstract

Hypothalamic dopamine inhibits pituitary prolactin secretion and proliferation of prolactin-producing lactotroph cells by activating lactotroph dopamine D2 receptors (D2Rs). Conversely, prolactin (PRL) stimulates hypothalamic dopamine neurons via PRL receptors (PRLRs) in a short-loop feedback circuit. We used Drd2-/- and Prlr-/- mutant mice to bypass this feedback and investigate possible dopamine-independent effects of PRL on lactotroph function. The absence of either receptor induced hyperprolactinemia and large prolactinomas in females. Small macroadenomas developed in aged Prlr-/- males, but only microscopic adenomas were found in Drd2-/- male mice. Pharmacologic studies in Prlr-/- mice with D2R agonists and antagonists demonstrated a significant loss of endogenous dopamine tone, i.e., constitutive inhibitory signaling by the D2R, in the pituitary. However, Prlr-/- mice exhibited more profound hyperprolactinemia and larger tumors than did age-matched Drd2-/- mice, and there were additive effects in compound homozygous mutant male mice. In vitro, PRL treatment markedly inhibited the proliferation of wild-type female and male Drd2-/- lactotrophs, but had no effect on female Drd2-/- lactotrophs, suggesting a downregulation or desensitization of PRLR in response to chronic hyperprolactinemia. We conclude that PRL inhibits lactotrophs by two distinct mechanisms: (a) indirectly by activation of hypothalamic dopamine neurons and (b) directly within the pituitary in a dopamine-independent fashion.

Original languageEnglish (US)
Pages (from-to)973-981
Number of pages9
JournalJournal of Clinical Investigation
Volume110
Issue number7
DOIs
StatePublished - Oct 2002

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Lactotrophs
Prolactin Receptors
Prolactinoma
Prolactin
Dopamine
Hyperprolactinemia
Dopaminergic Neurons
Dopamine Agents
Dopamine D2 Receptors
Adenoma
Down-Regulation

ASJC Scopus subject areas

  • Medicine(all)

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Lack of prolactin receptor signaling in mice results in lactotroph proliferation and prolactinomas by dopamine-dependent and -independent mechanisms. / Schuff, Kathryn; Hentges, Shane T.; Kelly, Michele A.; Binart, Nadine; Kelly, Paul A.; Michael Iuvone, P.; Asa, Sylvia L.; Low, Malcolm J.

In: Journal of Clinical Investigation, Vol. 110, No. 7, 10.2002, p. 973-981.

Research output: Contribution to journalArticle

Schuff, Kathryn ; Hentges, Shane T. ; Kelly, Michele A. ; Binart, Nadine ; Kelly, Paul A. ; Michael Iuvone, P. ; Asa, Sylvia L. ; Low, Malcolm J. / Lack of prolactin receptor signaling in mice results in lactotroph proliferation and prolactinomas by dopamine-dependent and -independent mechanisms. In: Journal of Clinical Investigation. 2002 ; Vol. 110, No. 7. pp. 973-981.
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